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Lab Meeting 10-18-13

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Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline Results of Single Colony That Grew on Tet + Caffeine Grown at 25C for 4 days

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Results: ecdm8 + TetA Tet Tet+ 0.1mM Caffeine Tet + 0.1mM Theophylline Grown at 25C for 4 days

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Drops of Theo or Caffeine on J303/4C5 + J79/1A2

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Disks of Theo or Caffeine on J303/1A2 + J79/4C5

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Disks of Theo or Caffeine on J303/4C5 + J79/1A2

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30 Degrees C

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Next Steps Continue Running Experiment at 30C for 2 days Finishing cloning ecdm8 downstream of TetA

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