Presentation is loading. Please wait.

Presentation is loading. Please wait.

Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E  Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno,

Published byCarla Arroyo Salazar Modified over 6 years ago

Similar presentations

Presentation on theme: "Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E  Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno,"— Presentation transcript:

1 Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E  Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno, Irene Molina-Martínez, José García-Solano, Miguel Perez-Guillermo, Pablo Conesa-Zamora  The Journal of Molecular Diagnostics  Volume 13, Issue 5, Pages (September 2011) DOI: /j.jmoldx Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 HRM analysis curves of specimens harboring heterozygous V600E (asterisk), WT V600 (empty arrow), and heterozygous R603X (solid arrow) detected by HRM as a non-V600E variant. RFU indicates relative fluorescence units. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 dHPLC curves obtained for different non-V600E mutations compared with the WT curve (arrows). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Comparison of the percentage of cases rendering no amplification for TaqMan (TM), dHPLC, HRM, and sequencing (Seq) techniques, according to the age of paraffin-embedded tissue. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5 Figure 4 Sensitivity test for the detection of the V600E mutation by dHPLC (A) and HRM (B). DNA from the V600E heterozygote cell line was serial diluted in DNA from the WT cell line. Percentages indicate the proportion of the V600E allele. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

6 Figure 5 Estimation of minimum cancer content harboring the V600E mutation by dHPLC and HRM. Percentages indicated are the estimated proportion of the V600E allele versus WT in the DNA extracted from paraffin-embedded tissue, as measured by dHPLC (A) and HRM (B). RFU indicates relative fluorescence units. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Download ppt "Comparison of Allelic Discrimination by dHPLC, HRM, and TaqMan in the Detection of BRAF Mutation V600E  Pablo Carbonell, María C. Turpin, Daniel Torres-Moreno,"

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics

KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics
The Use of COLD-PCR and High-Resolution Melting Analysis Improves the Limit of Detection of KRAS and BRAF Mutations in Colorectal Cancer  Irene Mancini,

The Use of COLD-PCR and High-Resolution Melting Analysis Improves the Limit of Detection of KRAS and BRAF Mutations in Colorectal Cancer  Irene Mancini,
Carol Beadling, Tanaya L. Neff, Michael C

Carol Beadling, Tanaya L. Neff, Michael C
Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia  Christopher.

Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia  Christopher.
Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification  Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.

Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification  Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.
Detection of Low-Level KRAS Mutations Using PNA-Mediated Asymmetric PCR Clamping and Melting Curve Analysis with Unlabeled Probes  Ji Eun Oh, Hee Sun.

Detection of Low-Level KRAS Mutations Using PNA-Mediated Asymmetric PCR Clamping and Melting Curve Analysis with Unlabeled Probes  Ji Eun Oh, Hee Sun.
KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics

KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics
Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA  Christina Wood-Bouwens, Billy T. Lau, Christine.

Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA  Christina Wood-Bouwens, Billy T. Lau, Christine.
A Method to Evaluate the Quality of Clinical Gene-Panel Sequencing Data for Single- Nucleotide Variant Detection  Chung Lee, Joon S. Bae, Gyu H. Ryu, Nayoung.

A Method to Evaluate the Quality of Clinical Gene-Panel Sequencing Data for Single- Nucleotide Variant Detection  Chung Lee, Joon S. Bae, Gyu H. Ryu, Nayoung.
Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,
Hendrikus J. Dubbink, Peggy N. Atmodimedjo, Ronald van Marion, Niels M

Hendrikus J. Dubbink, Peggy N. Atmodimedjo, Ronald van Marion, Niels M
A Quantitative Allele-Specific PCR Test for the BRAF V600E Mutation Using a Single Heterozygous Control Plasmid for Quantitation  Philippe Szankasi, N.

A Quantitative Allele-Specific PCR Test for the BRAF V600E Mutation Using a Single Heterozygous Control Plasmid for Quantitation  Philippe Szankasi, N.
Separate Quality-Control Measures Are Necessary for Estimation of RNA and Methylated DNA from Formalin-Fixed, Paraffin-Embedded Specimens by Quantitative.

Separate Quality-Control Measures Are Necessary for Estimation of RNA and Methylated DNA from Formalin-Fixed, Paraffin-Embedded Specimens by Quantitative.
Performance of Common Analysis Methods for Detecting Low-Frequency Single Nucleotide Variants in Targeted Next-Generation Sequence Data  David H. Spencer,

Performance of Common Analysis Methods for Detecting Low-Frequency Single Nucleotide Variants in Targeted Next-Generation Sequence Data  David H. Spencer,
A Multiplex SNaPshot Assay as a Rapid Method for Detecting KRAS and BRAF Mutations in Advanced Colorectal Cancers  Sandrine Magnin, Erika Viel, Alice.

A Multiplex SNaPshot Assay as a Rapid Method for Detecting KRAS and BRAF Mutations in Advanced Colorectal Cancers  Sandrine Magnin, Erika Viel, Alice.
Detection of CALR and MPL Mutations in Low Allelic Burden JAK2 V617F Essential Thrombocythemia  Fabrice Usseglio, Nathalie Beaufils, Anne Calleja, Sophie.

Detection of CALR and MPL Mutations in Low Allelic Burden JAK2 V617F Essential Thrombocythemia  Fabrice Usseglio, Nathalie Beaufils, Anne Calleja, Sophie.
Joanna Wang, Chetan Bettegowda  The Journal of Molecular Diagnostics 

Joanna Wang, Chetan Bettegowda  The Journal of Molecular Diagnostics 
Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus,

Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus,

Similar presentations

Ads by Google