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1 smart compact affordable
QIAxtractor smart compact affordable Brant Bassam PhD Andrea Tesoriero Application Specialist

2 QIAxtractor is a seamless integration to your workflow
post PCR analysis real time PCR or RT PCR PCR setup nucleic acid extraction Sample preparation Sample preparation DNA extraction aliquoting enzymatic digestion post purification QC enzymatic digestion PCR setup PCR & analysis

3 COMPACT QIAxtractor moves according to the experiment requirements
Fits into any work space and is easily movable Very small footprint Light and easily movable (25 kg; 51 lbs) Vacuum and waste stations located under the bench for more space and convenience 8-channel pipetting head without any tubing for more accuracy and easy maintenance Comes with a laptop for even more flexibility 8-Channel pipetting head

4 Bind-Elute Protocol Sample loaded with lysis buffer at 1:2 ratio
Lyse for 10 minutes Load the capture plate and apply vacuum Wash captured DNA with 1  Lysis Buffer 2  Propanol wash 1  Ethanolic wash Residual Ethanol is removed by vacuum Capture plate moves to the elution station Elution buffer loaded and vacuum applied DNA elutes into a 96-well cluster tube plate 4 4

5 Why automate? Minimize cross contamination risk Reduce human error
Reduce costs Increase throughput & save time Often the bottleneck in a project Work in increments of 8 samples (from 8 to 96) 96 samples in about 96 minutes Increase safety Minimum exposure to dangerous chemicals Versatility and choice DX liquid or tissue reagent, Vx reagent Wide range of protocols for DNA, RNA and viral nucleic acids Associated plasticware Maintain your sanity (pain threshold is about 25 extractions per week!) 5 5

6 Sample types successfully extracted
Blood -whole, fresh, frozen, buffycoat,Human, Mouse, Turtle, chickens, sheep Bovine Serum/Plasma, -HIV, Hep B , Hep C, CMV Urine Chlamydia and N. Gonorrhoea Swabs HSV1 and 2 Solid Tissue Mouse Tails, Prawn Tissue, Bird Quill Pulp Nasal Aspirates MPV (a single stranded RNA Virus) Cultured cells Micro Organisms Bacteria (Gram Pos. and Gram Neg.) and Fungi Plants Fruit, bark, leaves and stems Faeces Norris Virus Forensics cigarette butts, food scraps, finger prints, hair, fabric, FTA cards 6 6

7 QIAxtractor Easy to use system Low cost possible cost per extraction
Hands-free, walk-away operation High speed + high throughput (96 well) Consolidated chemistry minimum reagents and kit types work for all sample types Complete integrated system solution automation, reagents, consumables, protocols, options 7 7

8 Bench Top Unit 770 mm open 390 mm closed 490 mm 25 kg
Work surface enclosed by transparent cover 8-Channel pipetting head Dual position Manifold 1. Vacuum to Waste 2. Vacuum to Elute 1 2 Tip discard chute 4 SBS plate positions 3–8 Reagent Tubs (variable volumes) New photos New head design HEPA + UV in photo Add “compact” size 8 8

9 Under Bench Vacuum/Waste Station
To X-tractor Gene manifold Vacuum chamber coupling 19 kg 520 mm 495 mm 400 mm Bottle full sensor Pump coupling High power pump Carbon exhaust filter Waste bottle 4 L sanitary quick disconnect bottle PolyCap pump filter Vacuum sensor Software regulated -5 to -70 kPa, 80 L/min 9 9

10 Instrument Options UV Light HEPA filter
Irradiates surfaces between runs Robot arm moves so light reaches all areas Software controlled Timed post-run operation Must be factory fitted HEPA filter Positive pressure clean air supply Software controlled Integrated design Easy filter change Can be added after install New photo 10 10

11 CorProtocols™ for different applications
Available for web download Each comprises: PDF Protocol (Instructions for Use) X-tractor Gene Run File 11 11

12 For standard protocols simply:
Easy-to-use Wizard For standard protocols simply: 1. Click-select the columns to extract (in columns of 8) 2. Press Jump to End button and start run! 12 12

13 Software Intuitive color-coded interface mirrors actual deck layout
Active screen prompts For each reagent tub position, volumes needed are calculated for the number of samples to be extracted 13 13

14 Barcode Reader Compatible
Sample names and information can also be imported or exported Update 14 14

15 Pre- and Post-Run Reports
HTML report (universal format) Provides details of a run Documentation & Traceability (e.g. sample, batch and lot identifiers) Can be directly: Printed Saved ed Exported to Word 15 15

16 X-tractor Plasticware Pack (standard)
960 x OnCor™ C 200 µL Tips 5 x Square-well Lysis Blocks 60 x Elution Plate 8-Strip Caps 5 x Capture Plates 5 x Elution Plates (96 plain tube cluster rack) 5 x Sealing Films P/N 2270 Sufficient for 480 Extractions 16 16

17 OnCor™ C Tips rack design
SBS rack with replaceable lid for easy removal and storage between runs Total rack enclosure for security and contamination resistance Fully pocketed rack design Separate pockets for each tip prevents cross-contamination, allowing tip re-use during a run Tip re-use reduces costs Uses only 1.5 tip racks per 96 extractions P/N 2299 OnCor™ C 200 µL Fine Bore Tip 96 tips/rack, 10 racks/box 17 17

18 QIAxtractor minimizes cross-contamination risk Real-time amplification analysis of all samples
Real-Time Analysis Real-Time quantitative amplification analysis was done on all samples targeting the G6PD1 gene to single copy sensitivity (40 cycles) to test for inhibitors and for evidence of cross-contamination Amplification Results early, reproducible amp. of all samples no evidence of PCR inhibitors Cross-contamination Results no amplification of NTC samples no evidence of cross-contamination

19 QIAxtractor optimizes nucleic acid extraction yield Application in Veterinary Diagnostics
Viral detection of EHV-1 horses Dr. N. Pusterla at University of California, School of Veterinary Medicine Comparison of extraction efficiencies and DNA quality between manual and automated DNA extraction methods by real time analysis QIAxtractor (DX) Manual method (DNeasy) DNA concentration from blood aliquots (ug/100ul) [Absorbance at 260nm] 5.56 (+/- 3.98) 2.44 (+/- 1.21) Cycle threshold (Ct) values [threshold=0.1, baseline 3-15] 21.1 (0.4) 22.2 (0.5) Real time analysis evaluation of DNA quality: detection of the universal 18S rRNA housekeeping gene detection of EHV-1: amplification of the glycoprotein B (gB) gene Results significant higher concentrations of DNA recovered after automated extraction (p=0.001) significant stronger Ct values for the automated solution (p=0.01)

20 Rapid & sensitive detection of large numbers of specimens Application in Medical Diagnostics
Detection of noroviruses in fecal specimens Dr. J.A. Marshall at Victorian Infectious Diseases Reference Laboratory, Victoria (Australia) Experiment: total RNA extraction from fecal specimens + Analysis by multiplex RT PCR Manual: QIAamp viral RNA Manual: Roche high pure viral RNA Universal + QIAamp Virus Biorobot MDX QIAxtractor + VX chemistry Time for 36 specimens 2h - Time for 96 specimens 2h30 1h30 Time Operator was actually busy 25 min 15 min Mention: - that automation solutions save staff time for more engaging tasks - Universal performs at the same speed as QIAxtractor when same protocol / washing steps Results no significant difference between the manual and automation methods automation methods required less material automation methods process more samples in less time

21 Benefits Consistency & Workflow
Reduce human error and free-up technician time Standardisation Between labs and runs Documentation Pre- and Post-Run reports Safety No phenol/chloroform All waste safely contained for disposal Harmful fumes are carbon filtered out Work area completely enclosed by hood Run pauses if the hood is opened 21 21

22 SMART QIAxtractor is as convenient as QIAGEN can be
Fast 96 samples in 96 min Membrane tolerance: double flow rate; 4x more binding capacity compared to the other plates on the market Robust Just 2 chemistries (DX/VX) for NA extraction from virtually any sample type Consolidated chemistry for “liquids” and “tissues” Ultra-effective lyses buffer Easy Very intuitive and flexible software Just a few clicks of the mouse are enough to start a run Safe Innovative features: enclosed hood, UV light, HEPA filter … Optimal protection of the extraction and of the operator Individual elution tubes for easy downstream processing Re-use of tips results in little waste Membrane tolerance: double flow rate; 4x more binding capacity Membrane tolerance: double flow rate, 4x more binding capacity -> compared to what ?

23 Gracias por su atención

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