Presentation is loading. Please wait.

Presentation is loading. Please wait.

Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G.

Similar presentations


Presentation on theme: "Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G."— Presentation transcript:

1 Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G. Wilson, Ph.D., M.E. Levenston, Ph.D.  Osteoarthritis and Cartilage  Volume 16, Issue 10, Pages (October 2008) DOI: /j.joca Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 (A) Schematic of zone-specific articular chondrocyte harvest procedure. Osteochondral blocks were sectioned into three zones as shown. Areas shown in gray represent transitional sections between zones that were discarded. The area shown in red represents vascularized regions and calcified cartilage layers, which were also discarded. (B) Sketch of a fibrin hydrogel construct for oscillatory tensile loading studies. (C) Photograph of the oscillatory tensile loading device. [For intepretation of color in this figure, the reader is referred to the web version of the article.] Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 Characterization of zone-specific chondrocytes. (A) Initial cell diameter distribution prior to hydrogel seeding; data are mean±SD, n≥3800 cells. (B) Hydrogel DNA content normalized to day 3 values and (C) hydrogel sGAG content in free swelling culture; data are mean±s.e.m. ★ indicates significant difference from superficial; ‡ indicates significant difference from middle; P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

4 Fig. 3 Biosynthesis rates of zone-specific chondrocytes in fibrin hydrogel constructs subjected to intermittent oscillatory tensile loading; data are mean±s.e.m. ★ indicates significant difference from superficial; ‡ indicates significant difference from middle; indicates tension significantly different from unloaded; P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

5 Fig. 4 Total sGAG produced by zone-specific chondrocytes in fibrin hydrogel constructs subjected to intermittent oscillatory tensile loading; data are mean±s.e.m. Filled bars represent sGAG accumulated in the constructs, while open bars represent sGAG released to the culture medium. ★ indicates significant difference from superficial; ‡ indicates significant difference from middle; indicates tension significantly different from unloaded in all cases (construct, media, and total); indicates tension significantly different from unloaded only for release to culture medium; P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

6 Fig. 5 Analysis and quantification of proteoglycans and proteins released to the medium using size exclusion liquid chromatography. Data from (A, B) superficial zone constructs, (C, D) middle zone constructs, and (E, F) deep zone constructs are shown in rows and are the mean percentage of total CPM in each elution fraction for three replicates per group. Lines labeled I and II (A, C, E) designate peaks corresponding to large and small proteoglycans, respectively. Arrows (E, F) indicate the void and total volumes for the column. Quantitative data are shown as (G) the percentage of 35S-sulfate found in peak I or (H) the volume at which 50% of the total 3H-proline eluted from the column and are mean±s.e.m., n=3. ★ indicates significant difference from superficial; indicates tension significantly different from unloaded; P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

7 Fig. 6 Western blot analysis of peak II fractions containing proteoglycans released to the culture medium on the final day of culture. Upper, multiple aggrecan fragments bearing the G1 domain (∼65 and ∼90kDa); lower, multiple aggrecan fragments bearing the G3 domain (∼70 and ∼110kDa); U=unloaded and T=tension. Equal volumes of media were loaded in each lane. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions


Download ppt "Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G."

Similar presentations


Ads by Google