Presentation is loading. Please wait.

Presentation is loading. Please wait.

Sun A. Ham, Eun S. Kang, Hanna Lee, Jung S. Hwang, Taesik Yoo, Kyung S

Published bySamuel Beaudoin Modified over 6 years ago

Similar presentations

Presentation on theme: "Sun A. Ham, Eun S. Kang, Hanna Lee, Jung S. Hwang, Taesik Yoo, Kyung S"— Presentation transcript:

1 PPARδ Inhibits UVB-Induced Secretion of MMP-1 through MKP-7-Mediated Suppression of JNK Signaling 
Sun A. Ham, Eun S. Kang, Hanna Lee, Jung S. Hwang, Taesik Yoo, Kyung S. Paek, Chankyu Park, Jin-Hoi Kim, Dae-Seog Lim, Han G. Seo  Journal of Investigative Dermatology  Volume 133, Issue 11, Pages (November 2013) DOI: /jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Peroxisome proliferator–activated receptor (PPAR) δ inhibits UVB-induced secretion of matrix metalloproteinase (MMP)-1. (a–c) Human dermal fibroblasts transfected without (a) or with (b) small interfering RNA (siRNA) or pretreated with GSK0660 for 30minutes (c) were treated with GW for 24hours and then exposed to UVB. After incubation for 48hours, conditioned media were subjected to ELISA, zymography, or immunoblot analysis. (d) Cells pretreated with GW for 24hours were exposed to UVB. Following incubation for 24hours, a methyl thiazolyl tetrazolium assay was performed. GW and GSK0660 were dissolved in DMSO. Results are expressed as the means±SD (n=6). *P<0.01, **P<0.05 versus untreated group; #P<0.01, ##P<0.05 versus UVB-exposed group; ††P<0.05 versus UVB plus GW treated group. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Peroxisome proliferator–activated receptor (PPAR) δ suppresses UVB-induced reactive oxygen species (ROS) generation. Cells transfected with or without indicated small interfering RNA (siRNA) were pretreated with GSK0660 or N-acetyl-L-cysteine (NAC) for 30min and then treated with GW for 24hours. After exposure to UVB, the cells were incubated for 30minutes and then intracellular ROS levels were detected by (a) confocal laser fluorescence microscopy and (b, c) quantified. (d) Cells pretreated with NAC for 30minutes were treated with GW for 24hours and exposed to UVB. After incubation for 48hours, conditioned media were subjected to ELISA, zymography, or immunoblot analysis. Results are expressed as the means±SD (n=3 or 5). *P<0.01 versus untreated group; #P<0.01 versus UVB-exposed group; †P<0.01 versus UVB plus GW treated group. MMP-1, matrix metalloproteinase 1. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 c-Jun N-terminal kinase (JNK) signaling cascade is responsible for the peroxisome proliferator–activated receptor-mediated inhibition of matrix metalloproteinase (MMP)-1 in human dermal fibroblasts (HDFs) exposed to UVB. (a) HDFs were exposed to UVB for the indicated times. (b) Cells pretreated with GW for 24hours were exposed to UVB for 30minutes. An aliquot of protein was immunoblotted with activation-specific antibodies, and parallel immunoblots were analyzed for total kinase levels. (c) Cells were pretreated with SP600125, PD98059, or SB for 30minutes and then exposed to UVB. (d) Cells pretreated with SP for 30minutes were treated with GW for 24hours and then exposed to UVB. After incubation for 48hours, conditioned media were subjected to ELISA, zymography, or immunoblot analysis. Results shown are representative of three or four independent experiments. ERK, extracellular signal–regulated kinase. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Peroxisome proliferator–activated receptor δ attenuates UVB-induced activation of c-Jun N-terminal kinase (JNK) through the stabilization of mitogen-activated protein kinase phosphatase (MKP)-7. Human dermal fibroblasts (HDFs) pretreated with indicated doses of GW for 24hours were exposed to UVB irradiation for 30minutes. Cell extracts were separated by electrophoresis, (a) immunoblotted with the indicated antibodies, and (b) quantified. HDFs pretreated with 50nM GW (GW) for 24hours were exposed to UVB (40mJcm−2) for the indicated times in the presence of actinomycin D (Act D) (5μgml−1). (c) Total messenger RNA was extracted and subjected to northern blot analysis. (d) The radioactivity of the signals was quantified using an image analyzer; data are expressed as ratios of MKP-7 to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), normalized against the ratio at time zero. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Peroxisome proliferator–activated receptor δ prevents breakdown of type I and III collagen in UVB-irradiated human dermal fibroblasts (HDFs). HDFs pretreated with a range of GW for 24hours were exposed to UVB irradiation. Following incubation for 48hours, conditioned media or cell extracts were harvested and analyzed by (a) zymography or immunoblot analysis. Cells pretreated with (b) N-acetyl-L-cysteine (NAC) or (c) SP for 30minutes were incubated with GW for 24hours. Following exposure to UVB for 30minutes, the cells were incubated for 48hours. Cell extracts were analyzed by western blotting with the indicated antibodies. Representative images from three independent experiments are shown. MMP-1, matrix metalloproteinase 1. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Peroxisome proliferator–activated receptor δ prevents UVB-induced skin deformity in HR-1 hairless mice. (a–e) Representative cross-sections of the dorsal skin of UVB-exposed HR-1 hairless mice treated with or without GSK0660 and/or GW Wrinkle formation was detected by silicon replica assay (a). Paraffin tissue sections were subjected to hematoxylin and eosin (H&E) staining and quantitative analysis of epidermal and dermal thickness was performed (a, b). Bars=100μm. (c) Tissue extracts of dorsal skin from mice were subjected to immunoblot analysis. (d, e) Relative band intensities were quantified using an image analyzer and represented as a fold change relative to the untreated control. *P<0.01 versus untreated group; #P<0.01 versus UVB-exposed group; †P<0.01 versus UVB plus GW treated group. JNK, c-Jun N-terminal kinase; MKP, mitogen-activated protein kinase phosphatase; MMP-1, matrix metalloproteinase 1. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Sun A. Ham, Eun S. Kang, Hanna Lee, Jung S. Hwang, Taesik Yoo, Kyung S"

Similar presentations

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
SiRNA Knockdown of Tissue Inhibitor of Metalloproteinase-1 in Keloid Fibroblasts Leads to Degradation of Collagen Type I  Masayo Aoki, Koichi Miyake,

SiRNA Knockdown of Tissue Inhibitor of Metalloproteinase-1 in Keloid Fibroblasts Leads to Degradation of Collagen Type I  Masayo Aoki, Koichi Miyake,
Ginkgo biloba extract inhibits oxidized low-density lipoprotein (oxLDL)-induced matrix metalloproteinase activation by the modulation of the lectin-like.

Ginkgo biloba extract inhibits oxidized low-density lipoprotein (oxLDL)-induced matrix metalloproteinase activation by the modulation of the lectin-like.
Angela Tewari, Katarzyna Grys, Jutta Kollet, Robert Sarkany, Antony R

Angela Tewari, Katarzyna Grys, Jutta Kollet, Robert Sarkany, Antony R
Glutamine Suppresses DNFB-Induced Contact Dermatitis by Deactivating p38 Mitogen– Activated Protein Kinase via Induction of MAPK Phosphatase-1  Otgonzaya.

Glutamine Suppresses DNFB-Induced Contact Dermatitis by Deactivating p38 Mitogen– Activated Protein Kinase via Induction of MAPK Phosphatase-1  Otgonzaya.
Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes  Maryam G. Rohani, Brian K. Pilcher, Peter Chen,

Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes  Maryam G. Rohani, Brian K. Pilcher, Peter Chen,
Syk Mediates IL−17-Induced CCL20 Expression by Targeting Act1-Dependent K63- Linked Ubiquitination of TRAF6  Nan-Lin Wu, Duen-Yi Huang, Hsin-Ni Tsou, Ying-Cing.

Syk Mediates IL−17-Induced CCL20 Expression by Targeting Act1-Dependent K63- Linked Ubiquitination of TRAF6  Nan-Lin Wu, Duen-Yi Huang, Hsin-Ni Tsou, Ying-Cing.
Protective Role of Mitochondrial Peroxiredoxin III against UVB-Induced Apoptosis of Epidermal Keratinocytes  Jin Young Baek, Sujin Park, Jiyoung Park,

Protective Role of Mitochondrial Peroxiredoxin III against UVB-Induced Apoptosis of Epidermal Keratinocytes  Jin Young Baek, Sujin Park, Jiyoung Park,
Yeonsue Jang, Sang H. Jeong, Yoon-Hee Park, Hyun C

Yeonsue Jang, Sang H. Jeong, Yoon-Hee Park, Hyun C
Development of Cell-Penetrating Asymmetric Interfering RNA Targeting Connective Tissue Growth Factor  Jihye Hwang, Chanil Chang, Ji Hyun Kim, Chang Taek.

Development of Cell-Penetrating Asymmetric Interfering RNA Targeting Connective Tissue Growth Factor  Jihye Hwang, Chanil Chang, Ji Hyun Kim, Chang Taek.
Phytosphingosine Derivatives Ameliorate Skin Inflammation by Inhibiting NF-κB and JAK/STAT Signaling in Keratincoytes and Mice  Byung-Hak Kim, Ji Min.

Phytosphingosine Derivatives Ameliorate Skin Inflammation by Inhibiting NF-κB and JAK/STAT Signaling in Keratincoytes and Mice  Byung-Hak Kim, Ji Min.
Kynurenine Increases Matrix Metalloproteinase-1 and -3 Expression in Cultured Dermal Fibroblasts and Improves Scarring In Vivo  Yunyuan Li, Ruhangiz T.

Kynurenine Increases Matrix Metalloproteinase-1 and -3 Expression in Cultured Dermal Fibroblasts and Improves Scarring In Vivo  Yunyuan Li, Ruhangiz T.
Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes  Zheng Jun Li, Dae Kyoung Choi, Kyung Cheol Sohn, Min Seok Seo, Hae Eul Lee,

Propionibacterium acnes Activates the NLRP3 Inflammasome in Human Sebocytes  Zheng Jun Li, Dae Kyoung Choi, Kyung Cheol Sohn, Min Seok Seo, Hae Eul Lee,
Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.

Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.
P300 Is Elevated in Systemic Sclerosis and Its Expression Is Positively Regulated by TGF-β: Epigenetic Feed-Forward Amplification of Fibrosis  Asish K.

P300 Is Elevated in Systemic Sclerosis and Its Expression Is Positively Regulated by TGF-β: Epigenetic Feed-Forward Amplification of Fibrosis  Asish K.
Suppression of UV-Induced Wrinkle Formation by Induction of HSP70 Expression in Mice  Minoru Matsuda, Tatsuya Hoshino, Naoki Yamakawa, Kayoko Tahara,

Suppression of UV-Induced Wrinkle Formation by Induction of HSP70 Expression in Mice  Minoru Matsuda, Tatsuya Hoshino, Naoki Yamakawa, Kayoko Tahara,
Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.

Epidermal Growth Factor Induces Fibronectin Expression in Human Dermal Fibroblasts via Protein Kinase C δ Signaling Pathway  Yoshihiro Mimura, Hironobu.
Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 

Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 
Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.

Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.
Inhibitory Effects of Dietary Spirulina platensis on UVB-Induced Skin Inflammatory Responses and Carcinogenesis  Flandiana Yogianti, Makoto Kunisada,

Inhibitory Effects of Dietary Spirulina platensis on UVB-Induced Skin Inflammatory Responses and Carcinogenesis  Flandiana Yogianti, Makoto Kunisada,

Similar presentations

Ads by Google