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Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae  G. Tzanakaki,

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Presentation on theme: "Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae  G. Tzanakaki,"— Presentation transcript:

1 Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae  G. Tzanakaki, M. Tsopanomichalou, K. Kesanopoulos, R. Matzourani, M. Sioumala, A. Tabaki, J. Kremastinou  Clinical Microbiology and Infection  Volume 11, Issue 5, Pages (May 2005) DOI: /j x Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

2 Fig. 1 Examples of the results obtained when the multiplex PCR assay was applied to clinical samples, showing detection of Haemophilus influenzae type b (181 bp), Neisseria meningitidis (110 bp) and Streptococcus pneumoniae (80 bp). Lanes: 1, φX174 HaeIII digest; 2, positive control for all three targets; 3–13, clinical samples from blood (lanes 3, 6, 10, 11) or CSF (lanes 4, 5, 7, 8, 9, 12 and 13); 14, negative control. Clinical Microbiology and Infection  , DOI: ( /j x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions


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