1. Uptake of T-MPs for DC maturation and antigen presentation.
Uptake of T-MPs for DC maturation and antigen presentation. A, DCs were incubated with PKH67-labeled H22-MPs for 2, 6, or 12 hours, and the uptake of microparticles was detected by flow cytometry and fluorescent microscopy. Scale bars, 10 μm. Data are representative of three independent experiments. B, splenic T cells purified from H22 tumor–bearing mice were incubated with H22-MPs, DCs, or H22-MP–loaded DCs. T-cell proliferation was examined by CFSE dilution assay. Data are representative of three independent experiments. C, DC maturation was analyzed for expression of CD80, CD86, MHC II, and CCR7 by flow cytometry after coculture with H22-MPs for 36 hours. Data are representative of three independent experiments. D, 1 × 106 CFSE-labeled DCs or H22-MP–loaded DCs were injected into the footpads of syngeneic mice. Mice were sacrificed 24 or 48 hours after injection, and the number of CFSE DCs that migrated to the draining lymph nodes was analyzed. Data are representative of three independent experiments, and error bars represent mean ± SEM; *, P < E, mice were immunized with H22-MPs by footpad injection; 48 hours later, popliteal lymph nodes were dissected and surface expression of CD80, CD86, and MHC II of DCs was assessed by FACS. Data are representative of three independent experiments. F, IFNγ and IL12 of DCs in popliteal lymph nodes were analyzed by flow cytometry. Data are representative of three independent experiments, and error bars represent mean ± SEM; *, P < 0.05.
Huafeng Zhang et al. Cancer Immunol Res 2015;3:
©2015 by American Association for Cancer Research