Presentation is loading. Please wait.

Presentation is loading. Please wait.

A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.

Published byChristina Doyle Modified over 6 years ago

Similar presentations

Presentation on theme: "A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A."— Presentation transcript:

1 A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A. Bartley, Susan Latham, Bradley Budgen, David M. Ross, Elizabeth Hughes, Susan Branford, Deborah White, Timothy P. Hughes, Alexander A. Morley  The Journal of Molecular Diagnostics  Volume 17, Issue 2, Pages (March 2015) DOI: /j.jmoldx Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Comparison of minimal residual disease (MRD) measured by DNA real-time quantitative PCR (qPCR) and RT-qPCR. There were 193 samples in which MRD was detected by both RT-qPCR and DNA qPCR (closed circles), 50 samples in which MRD was detected only by DNA qPCR (open circles), no samples in which MRD was detected only by RT-qPCR, and eight samples in which MRD was not detected by either method. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Limit of detection (LOD) of quantitative RT-PCR (RT-qPCR). The percentage of samples detected was determined in a moving group of 15 samples and was taken to be the percentage detected at the median RT-qPCR value of the group. This percentage is related to the minimal residual disease (MRD) level measured by DNA qPCR on the same sample for which the median RT-qPCR had been determined. The dashed lines show the LOD, the value of which differs depending on the definition used. If LOD is the level at which 95% of positive samples are detected, which corresponds to three targets per assay, then the LOD of RT-qPCR is approximately 10−3.9. If LOD is the level at which one target is present, which corresponds to the level at which 63% of positive samples are detected, then the LOD of RT-qPCR is approximately 10−4.6. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Download ppt "A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A."

Similar presentations

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
Alexander A. Morley, Sue Latham, Michael J. Brisco, Pamela J

Alexander A. Morley, Sue Latham, Michael J. Brisco, Pamela J
Aberrant Levels of miRNAs in Bone Marrow Microenvironment and Peripheral Blood of Myeloma Patients and Disease Progression  Weixin Wang, Meghan Corrigan-Cummins,

Aberrant Levels of miRNAs in Bone Marrow Microenvironment and Peripheral Blood of Myeloma Patients and Disease Progression  Weixin Wang, Meghan Corrigan-Cummins,
Minimal Residual Disease Detection by Droplet Digital PCR in Multiple Myeloma, Mantle Cell Lymphoma, and Follicular Lymphoma  Daniela Drandi, Lenka Kubiczkova-Besse,

Minimal Residual Disease Detection by Droplet Digital PCR in Multiple Myeloma, Mantle Cell Lymphoma, and Follicular Lymphoma  Daniela Drandi, Lenka Kubiczkova-Besse,
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo.

Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo.
Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,
A Quantitative Allele-Specific PCR Test for the BRAF V600E Mutation Using a Single Heterozygous Control Plasmid for Quantitation  Philippe Szankasi, N.

A Quantitative Allele-Specific PCR Test for the BRAF V600E Mutation Using a Single Heterozygous Control Plasmid for Quantitation  Philippe Szankasi, N.
Chimerism Monitoring after Allogeneic Hematopoietic Stem Cell Transplantation Using Quantitative Real-Time PCR of Biallelic Insertion/Deletion Polymorphisms 

Chimerism Monitoring after Allogeneic Hematopoietic Stem Cell Transplantation Using Quantitative Real-Time PCR of Biallelic Insertion/Deletion Polymorphisms 
Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic.

Next-Generation Sequencing-Assisted DNA-Based Digital PCR for a Personalized Approach to the Detection and Quantification of Residual Disease in Chronic.
Whole-Genome Sequencing Identifies Patient-Specific DNA Minimal Residual Disease Markers in Neuroblastoma  Esther M. van Wezel, Danny Zwijnenburg, Lily.

Whole-Genome Sequencing Identifies Patient-Specific DNA Minimal Residual Disease Markers in Neuroblastoma  Esther M. van Wezel, Danny Zwijnenburg, Lily.
Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,

Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,
Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis  Elia Mattarucchi, Orietta.

Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis  Elia Mattarucchi, Orietta.
Detection of Cytomegalovirus in Whole Blood Using Three Different Real-Time PCR Chemistries  Erica Vincent, Zhengming Gu, Markus Morgenstern, Candace.

Detection of Cytomegalovirus in Whole Blood Using Three Different Real-Time PCR Chemistries  Erica Vincent, Zhengming Gu, Markus Morgenstern, Candace.
Adrian G. Selim, Andrew S. Moore  The Journal of Molecular Diagnostics 

Adrian G. Selim, Andrew S. Moore  The Journal of Molecular Diagnostics 
Joanna Wang, Chetan Bettegowda  The Journal of Molecular Diagnostics 

Joanna Wang, Chetan Bettegowda  The Journal of Molecular Diagnostics 

Similar presentations

Ads by Google