Presentation on theme: "Francis Zee 1, Lisa Keith 1, Amy Strauss 1, Claire Arakawa 1, Tristan Foote 1, Kim Hummer 2, Barbara Reed 2, Nahla Bassil 2, Stuart T. Nakamoto 3, Randall."— Presentation transcript:
Francis Zee 1, Lisa Keith 1, Amy Strauss 1, Claire Arakawa 1, Tristan Foote 1, Kim Hummer 2, Barbara Reed 2, Nahla Bassil 2, Stuart T. Nakamoto 3, Randall Hamasaki 3, Milton Yamasaki 3, Andrew Kawabata 3, Bob Durst 4, Allan K. Ikawa 5, Jodi Silva 5, Ken Love 6, Yongjian Chang 7
SCRI Project Goals Clonal propagation and production protocols Establish molecular fingerprinting and Cryopreservation methodology Conduct fruit nutrient analyses Determine host-pathogen interactions
SCRI Project Goals Produce ohelo as an ornamental from flask to nursery to market Prepare economic feasibility analyses of ohelo as ornamental potted plant Provide extension and outreach Develop seed based production protocols Initiate value added products and culinary research
Pélé Goddess of Fire Destroyed >100 structures since 1983 Added >70 acres to the SE coast
Goals Reduce wild gathering of ōhelo for commercial uses. Reduce traffic and the spread of invasive weeds Provide a sustainable (supply) of berries for culinary and value added product research (demand). Two hand- full of berries to start
Dr. Francis Zee and Vaccinium reticulatum seedlings
Ohelo Cultivar Releases 2009 Kilauea Nene Red Button
Microsatellite marker-based fingerprints for three important selections Nene Kilaua Red Button 294 308 294 296 294 298 Tested 23 blueberry (V. corymbosum) SSRs NA741: an example of one SSR that differentiates between 3 selections
SelectionsNA398CA794NA172VCC_I2CA421NA741NA1040NA800 Nene225, 229238, 242184210, 212191, 193294, 308192, 208198, 200, 208 Kilauea217, 221236, 238186, 188210193, 217294, 298194, 220198, 202, 206 Red Button213, 229238, 248182, 184210, 212195, 207294, 296208, 248198, 200 Microsatellite marker-based fingerprints for three important selections Eight SSRs reliably distinguish between these selections
Dendrogram based on microsatellite analysis using six primer pairs.
Section Myrtillus SSRs for 10 species in section Myrtillus Hawaiian taxa more recently derived Russian V. praestans grouped together Genetically identical V. myrtillus 1684-1 and 1684-3 from Kalmiopsis Wilderness, OR.
Germplasm conservation Sustainable germplasm resource for research Ornamental potted plants Alternate source from wild harvest Sustainable and reliable source of berry for culinary and value added product uses.
Diseases of Ōhelo Pucciniastrum vaccini Rust Calonectria sp. Leaf spots and sudden collapse - Calonectria sp. Powdery Mildew Microsphaera sp. 30 days after inoculation
Fruit Analysis Summary Anthocyanins Similar to cranberry, but v. low peonidin Rich source of phenolics Acids Similar profile to cranberry, but loweramounts Sugar profile Extremely Good source of PACs Especially V. calycinum
Pro Anthocyanadins SpeciesDMAC (mg-A2 equiv/100g) V. macrocarpon 125 V. reticulatum 117-146 V. calycinum 996-1183 10 x as much!
Ōhelo - Utilization Culinary recipes Ohelo Berry and Horseradish Pot Roast Sweettart Ohelo-Berry Compote Foie Gras with Ohelo Berries Goat Cheese and Ohelo Berry Bruschetta Grilled Lime Tequila Chicken with Ohelo Berry and Pineapple Sauce Ohelo and Oatmeal Pie Ohelo glazed Duck Ohelo creamed cheese pie
SCRI Project Goals Clonal propagation and production protocols Establish molecular fingerprinting Cryopreservation methodology Conduct fruit nutrient analyses Determine host-pathogen interactions
SCRI Project Goals Produce ohelo as an ornamental from flask to nursery to market Prepare economic feasibility analyses of ohelo as ornamental potted plant Provide extension and outreach Develop seed based production protocols Initiate value added products and culinary research SUCCESS!
Cooperators 1 U.S. Dept. Agriculture, Agricultural Research Service (USDA, ARS) Pacific Basin Agricultural Research Center (PBARC) P.O. Box 4487, Hilo, Hawaii 96720; 2 USDA ARS National Clonal Germplasm Repository (NCGR), 33447 Peoria Road, Corvallis, Oregon, 97333-2521; 3 University of Hawaii, College of Tropical Agriculture and Human Resources (UH, CTAHR), 1955East-West Rd. AgSci 314B,.Honolulu, HI 96822; 4 Linus Pauling Institute, Oregon State University, Corvallis, OR 97331-6512; 5 Research & Development, Big Island Candies, Hilo, HI; 6 Hawaii Tropical Fruit Grower Association, President and Agriculture Chair of the American Culinary Federation chapter – Kona Kohala Chefs Association, Kona, HI; 7 North American Plants LLC, McMinnville, OR 97128-8410.