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Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

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Presentation on theme: "Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C."— Presentation transcript:

1 Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C. Horsnell, PhD, Douglas A. Kuperman, PhD, Xiaozhu Huang, MD, David J. Erle, MD, Andreas L. Lopata, PhD, Frank Brombacher, PhD  Journal of Allergy and Clinical Immunology  Volume 126, Issue 2, Pages (August 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Airway resistance is not affected by loss of the IL-4Rα on smooth muscle cells. Airway resistance to increasing concentrations of intravenously administered acetylcholine (A), aerosolized methacholine (B), or Penh values in response to methacholine (C) were measured in IL-4Rα-/lox and SM-MHCcre IL-4Rα-/lox mice after OVA or PBS sensitization and challenge. Values represent pooled data with SD from 2 experiments with 4 to 8 mice per group. ∗P < .05; ∗∗∗P < .001 vs PBS control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Markers of lung inflammation are not affected by loss of the IL-4Rα on smooth muscle cells after sensitization and challenge with OVA. Total cell numbers (A) and numbers of eosinophils (Eos), neutrophils (Neu), lymphocytes (Lym), and macrophages (Mac) were counted in the BAL fluid. EPO (B) and mouse mast cell protease (mMCP-1; C) levels were measured in lung tissue lysate. Eotaxin concentration was measured in the blood serum by ELISA (D). Values represent pooled data with SD from 2 experiments with 4 to 6 mice per group. ∗P < .05; ∗∗P < .005; ∗∗∗P < .001 vs PBS control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Goblet cell hyperplasia and mucin production after sensitization and challenge with OVA is not affected by loss of the IL-4Rα on smooth muscle cells. Mucus-producing goblet cells were stained with PAS or anti-MUC5AC antibody in the airways of OVA–sensitized and challenged IL-4Rα-/lox(A), SM-MHCcre IL-4Rα-/lox(B), and IL-4Rα-/- mice (C) and PBS–sensitized and challenged IL-4Rα-/lox mice (D). Pictures were taken with a magnification of ×400, and representative examples of 3 experiments are shown here. Arrows indicate mucus-producing goblet cells. Aw, Airways. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Sensitization and challenge with OVA induces a Th2-type immune response in IL-4Rα-/lox and SM-MHCcreIL-4Rα-/lox mice. Lymphocytes (A) from mediastinal lymph nodes and splenocytes (B) were restimulated in vitro with OVA (100 μg/mL), and cytokine concentrations were measured in the supernatant by ELISA. ND, Not detected. ∗P < .05; ∗∗P < .005; ∗∗∗P < .001 vs IL-4Rα-/lox OVA. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 The allergen-specific antibody response of SM-MHCcreIL-4Rα-/lox mice resembles the antibody response of IL-4Rα-/lox mice. OVA-specific antibodies were measured by endpoint titration ELISA. Tested subclasses were IgE (A), IgG1(B), IgG2a(C), and IgG2b(D). Data represent pooled values with SD from 2 experiments with 4 to 6 mice per group. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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