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Demonstration of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms  Jukka Juvonen, MD, Tatu Juvonen, MD, PhD, Aino Laurila, MD, PhD, Hannu.

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Presentation on theme: "Demonstration of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms  Jukka Juvonen, MD, Tatu Juvonen, MD, PhD, Aino Laurila, MD, PhD, Hannu."— Presentation transcript:

1 Demonstration of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms 
Jukka Juvonen, MD, Tatu Juvonen, MD, PhD, Aino Laurila, MD, PhD, Hannu Alakärppä, MSc, Kari Lounatmaa, PhD, Heljä-Marja Surcel, PhD, Maija Leinonen, PhD, Matti I. Kairaluoma, MD, PhD, Pekka Saikku, MD, PhD  Journal of Vascular Surgery  Volume 25, Issue 3, Pages (March 1997) DOI: /S (97)70260-X Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Immunostaining of aneurysmal aortic wall with Chlamydia lipopolysaccharide-specific monoclonal antibody showing strong positive reaction in macrophages of atherosclerotic plaque (a) and in smooth muscle cells beneath plaque (b). No staining is seen in normal aorta (c). Bar 50 μm. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 1 Immunostaining of aneurysmal aortic wall with Chlamydia lipopolysaccharide-specific monoclonal antibody showing strong positive reaction in macrophages of atherosclerotic plaque (a) and in smooth muscle cells beneath plaque (b). No staining is seen in normal aorta (c). Bar 50 μm. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 1 Immunostaining of aneurysmal aortic wall with Chlamydia lipopolysaccharide-specific monoclonal antibody showing strong positive reaction in macrophages of atherosclerotic plaque (a) and in smooth muscle cells beneath plaque (b). No staining is seen in normal aorta (c). Bar 50 μm. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 2 Southern blotting of polymerase chain reaction products obtained with omp1-specific primers: Lane 1, Positive control (DNA extracted from C. pneumoniae strain Kajaani 6), lanes 2 and 3, two control aortas, and lanes 3 and 4, aneurysm tissues of two patients. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 3 Chlamydia-like particle in vessel wall of abdominal aortic aneurysm of two patients (a and b). OM, Outer membrane; CM, cytoplasmic membrane; PS, periplasmic space; CF, collagen fibers. Bar 0.2 μm. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 3 Chlamydia-like particle in vessel wall of abdominal aortic aneurysm of two patients (a and b). OM, Outer membrane; CM, cytoplasmic membrane; PS, periplasmic space; CF, collagen fibers. Bar 0.2 μm. Journal of Vascular Surgery  , DOI: ( /S (97)70260-X) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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