1. Measurement of cAMP-dependent protein kinase activity using a fluorescent-labeled Kemptide 
Lawrence J. Macala, John P. Hayslett, Joan I. Smallwood 
Kidney International 
Volume 54, Issue 5, Pages (November 1998)
DOI: /j x
Copyright © 1998 International Society of Nephrology Terms and Conditions

2. Figure 1
f-Kemptide phosphorylation by PKA catalytic subunit. (A) Linearity of f-Kemptide phosphorylation using purified bovine catalytic subunit. Values are the means of single determinations from two separate gels ± the range. Reactions were run for 20minutes at 30°C. (B) Phosphorylation of fluorescamine-labeled Kemptide compared to unlabeled Kemptide in the traditional [32P] assay, reported by Lutz et al1 (reprinted with permission). Symbols are: (•) gel-shift assay; (○) radioactive assay. (C) Phosphorylation of f-Kemptide in the present study (mean ± sd, N = 9).
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

3. Figure 2
f-Kemptide phosphorylation by A6 cell homogenate. (A) A UV-illuminated agarose gel of the products of reactions run with f-Kemptide and various amounts of homogenate protein. The upper band is phosphorylated f-Kemptide and the lower band is the remaining unphosphorylated peptide (lane a, 10 μg boiled homogenate protein; lanes b-e, 2.5, 5, 10 and 15 μg homogenate protein). Reactions were run for 15minutes at 30°C. (B) Concentration-dependence of measured A6 cell kinase activity in the presence of 1 μm exogenous cAMP. Values are the means of single determinations from each of two separate gels ± the range. Reactions were run for 20minutes at 30°C.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

4. Kidney International 1998 54, 1746-1750DOI: (10. 1046/j. 1523-1755
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions