1. Volume 119, Issue 6, Pages 1569-1579 (December 2000)
Peripheral corticotropin-releasing factor and stress-stimulated colonic motor activity involve type 1 receptor in rats 
Céline Maillot, Mulugeta Million, Jen Yu Wei, Ariane Gauthier, Yvette Taché 
Gastroenterology 
Volume 119, Issue 6, Pages (December 2000)
DOI: /gast
Copyright © 2000 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Dose-related stimulation of fecal pellet output induced by CRF, urocortin, and sauvagine injected IP in conscious nonfasted rats. Peptides (0.3–10 μg/kg) and saline (0.3 mL) were injected IP, and fecal pellet output was monitored 1 hour later (number/hour). Data are expressed as mean ± SEM in the number (n) of rats. *P < 0.05 vs. saline; †P < 0.05 vs. respective 0.3 μg/kg.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

3. Fig. 2
CRF, urocortin, and sauvagine injected IP induce clustered spike burst activity in cecum and proximal colon in nonfasted rats chronically implanted with electrodes. (A) Examples of recording trace before and 15 minutes after IP saline or peptides. (B) Percentage of clustered spike bursts from total spike burst activity per 15 minutes induced by IP injection of CRF-related peptides. Myoelectric activity in the cecum and proximal colon was monitored for 1 hour before (basal conditions) and after injection of saline or peptides. Bars represent mean ± SEM of 4–6 rats/group (1-way repeated-measures ANOVA). *P < 0.05 vs. basal and saline.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Astressin and CP-154,526 antagonize the increase of fecal pellet induced by IP CRF and water avoidance stress in conscious nonfasted rats. (A) Astressin (IP) and CP-154,526 (SC) were injected 15 and 30 minutes, respectively, before IP injection of saline (0.3 mL) or CRF (3 μg/kg), and pellet output was monitored 1 hour later (number/h). Bars represent the mean number of fecal pellets/h ± SEM of 5–9 rats/group (1-way ANOVA).*P < 0.05 vs. vehicle + saline, vs. CP-154,526 + saline, vs. astressin + saline. #P < 0.05 vs. vehicle + CRF. (B) Astressin (IP) and CP-154,526 (SC) were injected 15 and 30 minutes, respectively, before exposure to water avoidance stress (WAS) for 1 hour or no WAS, and pellet output was monitored 1 hour later (number/h). Bars represent the mean number of fecal pellets/h ± SEM of 5–11 rats/group (1-way ANOVA).*P < 0.05 vs. water + no WAS, vs. CP-154,526 + no WAS, vs. astressin + no WAS. #P < 0.05 vs. vehicle + WAS.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Astressin injected IP blocked IP CRF-induced clustered spike activity in conscious nonfasted rats. (A) Example of cecocolonic myoelectric activity under basal conditions and 15 minutes after IP injection of CRF in rats pretreated with either water or astressin. (B) Astressin blocked IP CRF-induced increase in percentage of clustered spike burst from total spike burst activity per 15 minutes. Myoelectric activity in the cecum and colon was monitored for 1 hour before (basal) and after IP injection of CRF with water or astressin pretreatment. Bars represent the mean ± SEM of 4 rats/group (1-way repeated-measures ANOVA). *P < 0.05 vs. basal; #P < 0.05 vs. vehicle + IP CRF.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 5
The CRF-R1 antagonist CP-154,526 blocked clustered spike burst activity induced by IP CRF in nonfasted rats chronically implanted with cecocolonic electrodes. (A) Example of cecocolonic myoelectric activity under basal conditions and 15 minutes after IP injection of CRF with vehicle or CP-154,526 pretreatment. (B) CP-154,526 blocked IP CRF-induced increase in percentage of clustered spike burst from total spike burst activity per 15 minutes in conscious nonfasted rats. Myoelectric activity in the cecum and colon was monitored for 1 hour before (basal) and after injection of CRF with water or CRF-R1 antagonist pretreatment. Bars represent the mean ± SEM of 4 rats/group (1-way repeated-measures ANOVA). *P < 0.05 vs. basal; #P < 0.05 vs. vehicle + IP CRF.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

7. Fig. 6
CRF injected into the inferior mesenteric artery increases the myoelectric activity of an in vitro isolated distal colon. Example of colonic myoelectric activity in vitro after intra-arterial injection of saline (0.1 mL) and CRF (600 ng). For each treatment, the upper trace shows colonic myoelectric activity and the lower trace displays the standard pulse generated thereof.
Gastroenterology  , DOI: ( /gast )
Copyright © 2000 American Gastroenterological Association Terms and Conditions