1. Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear export pathway.
Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear export pathway. (A) HeLa cells were cotransfected with expression vectors encoding EGFP‐MK5 and ERK3. After 24 h, cells were either left untreated (left panels) or exposed to LMB at a final concentration of 5 ng/ml for 4 h (right panels). Following fixation, EGFP‐MK5 was visualised directly (green channel in upper panels), ERK3 was visualised by staining with an anti‐ERK3 antibody and an Alexa 594‐coupled secondary (anti‐sheep) antibody (red channel in middle panels) and cell nuclei were visualised by DRAQ5 staining (blue channel in lower panels). Several fields of cells were examined and representative images are shown. (B) A total of 100 cells coexpressing both EGFP‐MK5 and ERK3 from each of three independent transfections were counted and the distribution of both proteins was scored. The results are presented as the % of cells in which both EGFP‐MK5 and ERK3 were either predominantly cytosolic (C>N) or predominantly nuclear (N>C) and mean values with associated errors are shown.
Ole‐Morten Seternes et al. EMBO J. 2004;23:
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