1. DHEA(S) and Allo induced the expression of the antiapoptotic Bcl-2 proteins in serum-deprived PC12 cells.
DHEA(S) and Allo induced the expression of the antiapoptotic Bcl-2 proteins in serum-deprived PC12 cells. Cells were cultured for 2–12 h either in complete or serum-free media containing 10–7 M DHEA, DHEAS, or Allo. Cellular extracts containing total mRNA or total proteins were collected, and levels of Bcl-2 and Bcl-xL mRNAs (A) and proteins (B) were measured either by semiquantitative RT-PCR or by Western blot, as described in Materials and Methods and normalized per actin mRNA or protein content, respectively. Values represent mean ± SD of three independent experiments (*, P < 0.05).
Ioannis Charalampopoulos et al. PNAS 2004;101:21:
©2004 by National Academy of Sciences