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Bacterial adherence to vascular prostheses

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1 Bacterial adherence to vascular prostheses
David D. Schmitt, M.D., Dennis F. Bandyk, M.D., Arch J. Pequet, M.D., Jonathan B. Towne, M.D.  Journal of Vascular Surgery  Volume 3, Issue 5, Pages (May 1986) DOI: / (86) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

2 Fig. 1 Scanning electron microscopy shows effect of washing and sonication on removal of Staphylococcus epidermidis (RP-12) organisms from surface of ePTFE graft (× 2000). A, Surface before nonadherent organisms are washed off. B, Surface after washing but before ultrasonic oscillation to dislodge adherent organisms, C, Graft surface after sonication with no organisms visible. Journal of Vascular Surgery 1986 3, DOI: ( / (86) ) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

3 Fig. 2 Quantitative bacterial adherence: Graft type variation. Data are expressed as number of colony-forming units per square centimeter of graft material per 107 inoculum; data in boxed areas compared for statistical significance, with inner boxes indicating statistically significant differences; p value determined by analysis of variance. ND = not determined. Journal of Vascular Surgery 1986 3, DOI: ( / (86) ) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

4 Fig. 3 Quantitative bacterial adherence: Species variation. Data are expressed as number of colony-forming units per square centimeter of graft material per 107 inoculum; data in boxed areas compared for statistical significance, with inner boxes indicating statistically significant differences; p value determined by analysis of variance. ND = not determined. Journal of Vascular Surgery 1986 3, DOI: ( / (86) ) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

5 Fig. 4 Scanning electron microscopy of adherent (A) mucin-producing RP-12, (B) nonmucin-producing SP-2 S. epidermidis, (C),S. aureus, and (D)E. coli microorganisms on ePTFE graft surface after 1 hour of incubation ( × 10,000). Morphology of attachment is similar. All strains attach as single bacterium, distinctly outlined. No evidence of extracellular glycocalyx production is visible. Journal of Vascular Surgery 1986 3, DOI: ( / (86) ) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

6 Fig. 5 Scanning electron microscopy of adherent mucin-producing (RP-12) microorganisms on velour Dacron graft surface after (A) 24 hours of incubation (× 5000) and (B) 48 hours of incubation (× 2000). RP-12 organisms are seen in greater numbers and form microcolonies buried in an amorphous ground substance produced by bacteria. Journal of Vascular Surgery 1986 3, DOI: ( / (86) ) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions


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