1. Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles 
Almudena Veiga-Lopez, D.V.M., Ph.D., Wen Ye, Ph.D., Vasantha Padmanabhan, Ph.D. 
Fertility and Sterility 
Volume 97, Issue 3, Pages (March 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Correlation between the degree of expression from categorical scale and mean density after quantification from individual follicles is shown for anti-Müllerian hormone (AMH; top), kit ligand (KL; middle), and growth differentiation factor 9 (GDF9; bottom). Pearson correlation coefficient is shown as R2.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
(Top) Representative negative and positive control images from anti-Müllerian hormone (AMH; left), kit ligand (KL; middle), and growth differentiation factor 9 (GDF9; right) immunostaining in large preantral follicles from 10-month-old ovaries. (Bottom) Representative images of AMH (left columns), KL (middle columns), and GDF9 (right columns) immunostaining in primordial, primary, small preantral, large preantral, small antral, and large antral follicles in 10-month-old control and prenatal T-treated females. Bar = 50 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) Percentage of follicles that expressed growth differentiation factor 9 (GDF9) at fetal ages day (D) 90 and D140. (B) Impact of prenatal T on expression of anti-Müllerian hormone (AMH), kit ligand (KL), and GDF9 in ovaries of prepubertal (top; 5.5-month-old), postpubertal (middle; 10-month-old), and adult (bottom; 21-month-old) sheep (control: open bars; prenatal T-treated: closed bars) sheep. Data expressed as mean ± SEM. Significant differences between treatment groups within follicle class are indicated by asterisks (∗P<.05). Significant differences between follicle class within marker and age are indicated by different letters (a ≠ b; P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Schematic summarizing direction of changes in anti-Müllerian hormone (AMH) expression across follicular stages and the impact of prenatal T excess. During normal follicular differentiation (left), AMH levels increase from primordial to the preantral stage and are implicated in suppression of follicular recruitment. The AMH expression is reduced in large antral follicles, thus contributing to increased follicular FSH sensitivity. Prenatal T (right) reduces expression of AMH in preantral follicles, thus contributing to increased follicular recruitment. Lack of AMH suppression in large antral follicles of prenatal T-treated animals may reduce FSH sensitivity and thus contributing toward arrested follicular growth and persistence. Gr = granulosa cells; Oc = oocyte; Th = theca cells.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions