1. Synthetic view of genome‐wide mRNA profile changes induced by toxic GSH levels and GSH depletion.
Synthetic view of genome‐wide mRNA profile changes induced by toxic GSH levels and GSH depletion. Genes affected by GSH at toxic levels (HGT1 cells cultured with 50 μM GSH during 30 min), and by GSH depletion (gsh1Δ cells grown for three and six divisions in medium devoid of GSH) identified by DNA microarray analysis (see Supplementary Tables S2 and S3), in duplicate independent cultures, and assigned to functional categories ( Numbers indicate the sum of regulated genes in each functional category. Genes represented comprise 61% (228/375) of the total genes regulated by GSH toxic levels and 68% (121/178) of the total genes regulated by GSH depletion. A few genes are present in more than one category. Data of transcriptional responses to ATM1 depletion (Gal‐ATM1) were from Hausmann et al (2008), to iron deficiency from Puig et al (2005) and to DTT/tunicamycin (UPR) from Travers et al (2000) and Kimata et al (2006).
Chitranshu Kumar et al. EMBO J. 2011;30:
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