1. Early Onset of Immunoglobulin Heavy Chain Gene Rearrangements in Normal Human Bone Marrow CD34+ Cells
by Frédéric Davi, Ahmad Faili, Catherine Gritti, Catherine Blanc, Caroline Laurent, Laurent Sutton, Christian Schmitt, and Hélène Merle-Béral
Blood
Volume 90(10):
November 15, 1997
©1997 by American Society of Hematology

2. Isolation of CD34+ CD19+ and CD34+ CD19− populations by cell sorting.
Isolation of CD34+ CD19+ and CD34+ CD19− populations by cell sorting. Low density mononuclear cells from adult BM were labeled with anti-CD34–FITC and anti-CD19–PE, and cells within the lymphoid blast window were analyzed before or immediately after double cell sorting on a FACStar Plus flow cytometer. The purity of the sorted cells in each fraction was at least 99%.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology

3. Detection of VDJH rearrangements in BM cell fractions.
Detection of VDJH rearrangements in BM cell fractions. DNA isolated from purified cell fractions (A) or dilutions of CD34− CD19+ B cells in keratinocytes (B) were amplified with consensus FR3 and JH primers. PCR products were subjected to Southern blot hybridization using an internal consensus JH oligonucleotide probe. Amplification of the RAG-1 gene served as control for DNA integrity.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology

4. Detection of partial DJH rearrangements in BM cell fractions.
Detection of partial DJH rearrangements in BM cell fractions. DNA from cell lysates was amplified with a mixture of consensus primers for most of DH gene families and a consensus JH primer. PCR products were visualized by Southern blot hybridization using an internal consensus JH probe. GL DHQ52 indicates the amplification, from a germline IgH locus, of the DNA segment located between the most 3′ DH gene (DHQ52 ) and the most 5′ JH gene (JH1 ), producing a 160-bp product.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology

5. Three-color fluorescence analysis of BM cells labeled with anti-CD34–FITC, anti-CD10–PE, and anti-CD19–APC.
Three-color fluorescence analysis of BM cells labeled with anti-CD34–FITC, anti-CD10–PE, and anti-CD19–APC. This representative experiment shows the existence of a CD34+ CD10+ CD19− population. Boxes on the right panel represent the window gates used during the sort procedure.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology

6. Detection of partial DJH rearrangements in BM CD34+ fractions.
Detection of partial DJH rearrangements in BM CD34+ fractions. Amplification of the germline DHQ52-JH1 DNA segment (GL DHQ52 ) served as an internal control for DNA integrity.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology

7. Model of human early B-cell differentiation.
Model of human early B-cell differentiation. LSC, lymphoid stem cell; GL, germline.
Frédéric Davi et al. Blood 1997;90:
©1997 by American Society of Hematology