1. Xer recombination at variant sites.
Xer recombination at variant sites. (A) Sequence comparison of the core recombination sites dif, dib, cer6 and psi. All sites possess a 6 bp central region (bold) flanked by 11 bp XerC‐ and XerD‐binding sites. For dib, cer6 and psi only nucleotides that distinguish these sites from dif are shown. (B) Recombination at variant sites. Plasmid DNA was isolated from Xer+ FtsK+ (DS941), FtsKc− (DS9041) and XerC− (DS981) strains. Bands running behind the substrates (S) represent multimeric forms of the plasmids. In the case of the plasmid containing the cer6 site without accessory sequences, Xer‐mediated resolution generates two product circles (RP) of similar size, with the larger circle being competent to replicate. (C) Recombination in dif‐ and dib‐containing plasmids. Strains were as described in (B). S1 and S2 represent the dif‐containing (pSDC124) and dib‐containing (pOW4) substrates, respectively. The resolution products (RP) generated by Xer recombination within both of these plasmids are identical in size. SS and RPS indicate supercoiled plasmids; SL and RPL denote NdeI‐restricted plasmids. Note the incomplete digestion of the dif‐containing plasmid from the FtsK+ strain.
Gavin D. Recchia et al. EMBO J. 1999;18:
© as stated in the article, figure or figure legend