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Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis.

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Presentation on theme: "Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis."— Presentation transcript:

1 Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis C Virus Analyte-Specific Reagent  Michael S. Forman, Alexandra Valsamakis  The Journal of Molecular Diagnostics  Volume 10, Issue 2, Pages (March 2008) DOI: /jmoldx Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Measurable range (IU/ml) of different HCV genotypes by CAP TNAI/CTM48. A: Gt 1. B: Gt 2. C: Gt 3. Dashed line indicates theoretical trend line of complete agreement between expected and observed values. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Quantitative agreement between two different extraction methods and two different quantitative methods: combined analysis of all samples. A: Quantitative correlation between CAP TNAI/CTM48 and M48/CTM48 (Gt 1, n = 61; Gt 2, n = 27; Gt 3, n = 16; Gt 4, n = 11; Gt unknown, n = 6). B: Difference in quantification between CAP TNAI/CTM48 and M48/CTM48. Mean, 0.00; SD, 0.22 log10 IU/ml. C: Quantitative correlation between CAP TNAI/CTM48 and CAM (Gt 1, n = 49; Gt 2, n = 23, Gt 3, n = 15; Gt 4, n = 11). D: Difference in quantification between CAP TNAI/CTM48 and CAM. Mean, −0.27 log10 IU/ml; SD, Dashed line in A and C indicates theoretical trend line of complete agreement. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Quantitative agreement between two different extraction methods: separate analyses of Gt 1 and non-Gt 1 samples. A: Quantitative correlation between CAP TNAI/CTM48 and M48/CTM48 for Gt 1 samples (n = 61). B: Difference in quantification between CAP TNAI/CTM48 and M48/CTM48 for Gt 1 samples. Mean, −0.04; SD, C: Quantitative correlation between CAP TNAI/CTM48 and M48/CTM48 for Gt non-1 samples (Gt 2, n = 27; Gt 3, n = 16; Gt 4, n = 11). D: Difference in quantification between CAP TNAI/CTM48 and M48/CTM48 for non-Gt 1 samples. Mean, 0.03; SD, Dashed line in A and C indicates theoretical trend line of complete agreement. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 Quantitative agreement between two different quantification methods: separate analyses of Gt 1 and non-Gt 1 samples. A: Quantitative correlation between CAP TNAI/CTM48 and CAM for Gt 1 samples (n = 49). B: Difference in quantification between CAP TNAI/CTM48 and CAM for Gt 1 samples. Mean, −0.33; SD, C: Quantitative correlation between CAP TNAI/CTM48 and CAM for Gt non-1 samples (Gt 2, n = 23; Gt 3, n = 15; Gt 4, n = 11). D: Difference in quantification between CAP TNAI/CTM48 and CAM for non-Gt 1 samples. Mean, −0.22; SD, Dashed line in A and C indicates theoretical trend line of complete agreement. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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