Presentation is loading. Please wait.

Presentation is loading. Please wait.

Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H

Published byMaximilian Poole Modified over 5 years ago

Similar presentations

Presentation on theme: "Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H"— Presentation transcript:

1 Ginkgolide A attenuates homocysteine-induced endothelial dysfunction in porcine coronary arteries 
Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H. Lin, MD, Alan B. Lumsden, MD, Qizhi Yao, MD, PhD, Changyi Chen, MD, PhD  Journal of Vascular Surgery  Volume 44, Issue 4, Pages (October 2006) DOI: /j.jvs Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Effects of homocysteine (Hcy) and ginkgolide A (GA) on vasomotor function in porcine coronary arteries. A, Contractility. The rings (eight per group) were cultured in dimethyl sulfoxide (DMSO) control, homocysteine (50 μmol/L) alone, low-dose GA (50 μmol/L) alone, or high-dose GA (100 μmol/L) alone and homocysteine combined with low-dose or high-dose GA. There was no substantial difference in maximum vessel contraction in response to U46619 (10−7 mol/L) among all groups (P > .05). B, Endothelium-dependent relaxation. Vasorelaxation in response to cumulative doses of bradykinin demonstrated improved vasorelaxation among all groups as the concentration of bradykinin increased. Both the low-dose and high-dose GA/homocysteine groups showed significant improvements in vasorelaxation compared with homocysteine alone at the bradykinin concentrations of 10−7, 10−6, and 10−5 mol/L. C, Vasorelaxation in response to the highest dose of bradykinin (10−5 mol/L). The homocysteine groups showed significant impairments in vasorelaxation as compared with the control (P < .05), whereas both the low-dose and high-dose GA/homocysteine groups demonstrated a significant improvement in vasorelaxation as compared with homocysteine (P < .05) and were similar to the control group. D, Endothelium-independent relaxation. There was no significant difference among all groups (P > .05) in the endothelium-independent vasorelaxation induced by sodium nitroprusside. EC, endothelial cells. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Effects of homocysteine (Hcy) and ginkgolide A (GA) on endothelial nitric oxide synthase (eNOS) messenger RNA (mRNA) levels in porcine coronary arteries. The levels of eNOS mRNA expression in porcine coronary arteries were measured by using real-time polymerase chain reaction analysis. The relative expression for eNOS in each sample was normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The homocysteine-alone group showed a marked reduction in eNOS mRNA levels as compared with the control, and GA dose-dependently improved eNOS mRNA levels in the GA/homocysteine groups. DMSO, Dimethyl sulfoxide. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Effects of homocysteine and ginkgolide A (GA) on endothelial nitric oxide synthase (eNOS) messenger RNA (mRNA) levels in porcine coronary artery endothelial cells (PCAECs). The levels of eNOS mRNA expression in PCAECs were measured by using real-time polymerase chain reaction analysis. The relative expression for eNOS in each sample was normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The homocysteine-alone group showed a marked reduction in eNOS mRNA levels as compared with the control, and GA dose-dependently improved eNOS mRNA levels in the GA/homocysteine groups. GA alone did not show any effect on eNOS expression. DMSO, Dimethyl sulfoxide. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Effects of homocysteine (Hcy) and ginkgolide A (GA) on endothelial nitric oxide synthase (eNOS) immunoreactivity in porcine coronary arteries. A, The dimethyl sulfoxide (DMSO) control group showed a strong positive staining pattern of eNOS on the endothelial layer of the arterial wall. B, The homocysteine-alone group showed a poor staining pattern of eNOS. The low-dose and high-dose GA-alone groups (C-F) and the low-dose and high-dose GA/homocysteine combined groups (E-F) showed a strong positive staining pattern similar to the control group. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 Effects of homocysteine (Hcy) and ginkgolide A (GA) on nitric oxide production in porcine coronary artery endothelial cells. Cells were stained with the fluorescent dye 3-Amino, 4-aminomethyl-2’, 7’-difluorofluorescein Diacetate (DAF-FM DA) and analyzed by using flow cytometry assay. A, Representative histograms of the flow cytometry assay. B, Percentages of DAF-FM DA–staining positive cells. The homocysteine-alone group showed a 26% decrease in the nitric oxide level as compared with control, and GA at a 10, 20, 50, or 100 μmol/L concentration effectively reversed the homocysteine-induced nitric oxide decrease (P < .05). GA at 1 μmol/L did not reverse the homocysteine-induced nitric oxide decrease. C, Mean fluorescence intensity of DAF-FM DA–staining positive cells. The homocysteine-alone group showed a 38% decrease in the nitric oxide level as compared with control, and GA at 50 or 100 mmol/L effectively reversed the homocysteine-induced nitric oxide decrease (P < .05). GA at 1, 10, or 20 μmol/L did not significantly reverse the homocysteine-induced nitric oxide decrease. DMSO, Dimethyl sulfoxide. FL1-H, a fluorescence channel used in flow cytometry. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

7 Fig 6 Effects of homocysteine (Hcy) and ginkgolide A (GA) on superoxide anion production in porcine coronary arteries. Superoxide anion was markedly increased in the homocysteine-treated groups as compared with the dimethyl sulfoxide (DMSO) control (P < .05). High-dose or low-dose GA alone showed no effects on the level of peak chemiluminescence as compared with the control. Both the high-dose and low-dose GA/homocysteine combined groups showed a substantial reduction in superoxide anion levels. RLU, Relative light unit. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Download ppt "Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H"

Similar presentations

Volume 84, Issue 1, Pages (July 2013)

Volume 84, Issue 1, Pages (July 2013)
The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent 

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent 
Modulation of growth in human esophageal adenocarcinoma cells by group IIa secretory phospholipase A2  David Mauchley, MD, Xianzhong Meng, MD, PhD, Thomas.

Modulation of growth in human esophageal adenocarcinoma cells by group IIa secretory phospholipase A2  David Mauchley, MD, Xianzhong Meng, MD, PhD, Thomas.
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,

Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,
Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.

Early hypercholesterolemia contributes to vasomotor dysfunction and injury associated atherogenesis that can be inhibited by nitric oxide  Kathleen G.
Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells 

Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells 
Bone Morphogenic Protein-4 Contributes to Venous Endothelial Dysfunction in Patients With Diabetes Undergoing Coronary Revascularization  Jia Hu, MD,

Bone Morphogenic Protein-4 Contributes to Venous Endothelial Dysfunction in Patients With Diabetes Undergoing Coronary Revascularization  Jia Hu, MD,
Endothelial cells are susceptible to rapid siRNA transfection and gene silencing ex vivo  Nicholas D. Andersen, MD, Atish Chopra, Thomas S. Monahan, MD,

Endothelial cells are susceptible to rapid siRNA transfection and gene silencing ex vivo  Nicholas D. Andersen, MD, Atish Chopra, Thomas S. Monahan, MD,
Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P

Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P
Reactive oxygen species mediate functional differences in human radial and internal thoracic arteries from smokers  Else Müller-Schweinitzer, MD, Sandra.

Reactive oxygen species mediate functional differences in human radial and internal thoracic arteries from smokers  Else Müller-Schweinitzer, MD, Sandra.
Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries  Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,

Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries  Birgit Zatschler, Peter Dieterich, PhD, Bianca Müller,
Endothelial nitric oxide synthase enhancer for protection of endothelial function from asymmetric dimethylarginine–induced injury in human internal thoracic.

Endothelial nitric oxide synthase enhancer for protection of endothelial function from asymmetric dimethylarginine–induced injury in human internal thoracic.
Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,
Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling  Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling  Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,
Hypercholesterolemia Impairs the Myocardial Angiogenic Response in a Swine Model of Chronic Ischemia: Role of Endostatin and Oxidative Stress  Munir Boodhwani,

Hypercholesterolemia Impairs the Myocardial Angiogenic Response in a Swine Model of Chronic Ischemia: Role of Endostatin and Oxidative Stress  Munir Boodhwani,
Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 

Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 
Low molecular weight (LMW) heparin inhibits injury-induced femoral artery remodeling in mouse via upregulating CD44 expression  Gaofeng Zhao, MD, PhD,

Low molecular weight (LMW) heparin inhibits injury-induced femoral artery remodeling in mouse via upregulating CD44 expression  Gaofeng Zhao, MD, PhD,
Acute limb ischemia secondary to myositis-induced compartment syndrome in a patient with human immunodeficiency virus infection  Russell Lam, MD, Peter.

Acute limb ischemia secondary to myositis-induced compartment syndrome in a patient with human immunodeficiency virus infection  Russell Lam, MD, Peter.
David G. Cable, MD, James A. Caccitolo, MD, Eric A

David G. Cable, MD, James A. Caccitolo, MD, Eric A

Similar presentations

Ads by Google