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Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

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Presentation on theme: "Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling."— Presentation transcript:

1 Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling Chen, MD, Nan Wang, MD, PhD, Zhi-Chao Wang, MD, Jin Ma, MD, PhD, Rong-Fei Zhu, MD, PhD, Xiao-Yan Xu, MD, Peng-Cheng Zhou, PhD, Di Yu, PhD, Zheng Liu, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 142, Issue 1, Pages e10 (July 2018) DOI: /j.jaci Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Increased numbers of circulating TFH2 cells in patients with AR. A, Percentages of circulating non-TFH and TFH cells among total CD4+ T cells in patients with AR (n = 27) and HC subjects (n = 28). B, Percentage of cell subsets among non-TFH and TFH cells in patients with AR (n = 27) and HC subjects (n = 28). C, TFH and non-TFH cells were cocultured with autologous naive B cells, and immunoglobulin levels were detected at day 8 (n = 8). D, Non-TFH and TFH cell subsets were cocultured with autologous naive B cells, and IgE levels were detected at day 8 (n = 6). E, TFH2 cells from patients with AR were cocultured with autologous naive B cells in the presence of blocking antibody or protein, and IgE levels were detected at day 8 (n = 6). In Fig 1, A and B, data are presented as means ± SDs. In Fig 1, C-E, data are presented as means ± SEMs. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Decrease in numbers of circulating allergen-specific IL-4+CXCR5+CD4+ TFH cells after AIT. A, Correlations between frequencies of Der p 1–specific IL-4+ non-TFH and TFH cells with D pteronyssinus–specific IgE levels in patients with AR. B, Changes in percentages of Der p 1–specific IL-4+ cells within non-TFH and TFH cells in patients with AR with (n = 22) and without (n = 20) AIT after treatment. Data are presented as means ± SDs. C, Correlations of reduction in numbers of Der p 1–specific IL-4+ non-TFH and TFH cells with CSMS improvement in patients with AR with and without AIT after treatment. *P < .05 and ***P < .001. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Peripheral TFH and non-TFH cells among total CD4+ T cells. A, Gating strategy for CXCR5− non-TFH cells and CXCR5+ TFH cells and their subsets in peripheral blood. FSC, Forward scatter; FV700, fixable viability stain 700; SSC, side scatter. B, Percentages of subsets of TFH and non-TFH cells in total CD4+ T cells (n = 28 for HC subjects and n = 27 for patients with AR). Data are presented as means ± SDs. *P < .05. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Immunoglobulin-inducing function of circulating TFH and non-TFH cells. A, Non-TFH and TFH cells from HC subjects or patients with AR were cocultured with autologous naive B cells, and immunoglobulin levels were detected at day 8 (n = 8). B, TFH and non-TFH cell subsets from HC subjects or patients with AR were cocultured with autologous naive B cells, and immunoglobulin levels were detected at day 8 (n = 6). C, TFH2 cells from patients with AR were cocultured with autologous naive B cells, and cytokine levels were detected at day 2 (n = 6). Data are presented as means ± SEMs. **P < .01. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Allergen-specific circulating TFH cells in patients with AR. A, Carboxyfluorescein succinimidyl ester (CFSE)–labeled circulating CXCR5− non-TFH and CXCR5+ TFH cells were cultured with autologous monocytes, which were pretreated with Der p 1 or staphylococcal enterotoxin B. Cell proliferation was analyzed by using flow cytometry at day 7 (n = 6). Representative histograms are shown. Data are expressed as means ± SEMs. B, Percentages of Der p 1–specific IL-4+ non-TFH and TFH cells within corresponding total non-TFH and TFH cells. Representative flow cytometric plots are shown. Data are presented as means ± SDs (n = 27 for patients with AR and n = 25 for HC subjects). *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 Changes in immunoglobulin levels and TH cell counts in patients with AR with or without subcutaneous AIT after treatment. A, Changes in CSMS. Data are expressed as means ± SDs. B, Changes in total IgE, D pteronyssinus–specific IgE, and D pteronyssinus–specific IgG4 levels. Data are expressed as means ± SEMs. C-E, Changes in TH cell (Fig E4, C), CXCR5− non-TFH cell subset (Fig E4, D), and CXCR5+ TFH cell subset counts (Fig E4, E; n = 22 for patients with AIT and n = 20 for patients without AIT). F, Correlations of reductions in D pteronyssinus–specific IgE levels with improvement in CSMS and decrease in Der p 1–specific IL-4+ TFH cell counts. Data are expressed as means ± SDs. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E5 Patterns of chemokine receptor expression in Der p 1–specific IL-4+ TFH and Der p 1–specific IL-4+ non-TFH cells in patients with AR. Representative histograms are shown. Numbers in figures refer to percentages of positive cells (n = 2). Journal of Allergy and Clinical Immunology  , e10DOI: ( /j.jaci ) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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