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Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Safety Rules for Medical Biochemistry Students 1.

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Presentation on theme: "Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Safety Rules for Medical Biochemistry Students 1."— Presentation transcript:

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2 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Safety Rules for Medical Biochemistry Students 1

3 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 2 Welcome To The Biochemistry Lab Always remember every time: To Be on time To record your attendance by your ID To Get Your White Coat To Get Your Student recordrecord To be creative ! To follow your contract itemscontract

4 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 3

5 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 4

6 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 5

7 Principle Remember that the lab is a place for serious work! Careless behavior may endanger yourself and others and will not be tolerated! 6 ILO: By the end of this topic, the student will be able to: Practice basics of safety procedures and apply the different general rules of safety during learning in Lab.

8 Purpose Provide guidance and protocols for the protection of students from laboratory hazardous materials. 7

9 Why does it matter? Safe working protects: – You – Other lab workers – Cleaners – Visitors – Your work 8

10 What are the general hazards in a laboratory? Fire Breakage of glassware Sharps Spillages Pressure equipment & gas cylinders Extremes of heat & cold Chemical hazards Biological hazards Radiation And many more! 9

11 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Appearance and attitude in the lab. General work procedure. Hygiene practice. Emergency procedure. Safety Rules for Medical Biochemistry Students includes : 10

12 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Appearance and attitude in the lab While working in the lab, the following instructions should be followed: 11

13 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department You must wear your clean white coat يجب ارتداء البالطو الابيض الخاص بك 12

14 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Do not use any flammable materials such as perfumes. لا تستعمل المواد القابله للاشتعال مثل البرفانات Tie up long hair during laboratory activity يجب عدم ترك الشعر منسدلا اثناء العمل فى المعمل You must insert the jewelry and veil into the coat يجب ادخال الاكسسوار اوالحجاب فى داخل البالطو

15 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 14 Try always to wear leather shoes that cover your feet. حاول دائما ارتداء حذاء جلدى يغطي القدمين Sandals and open-toed shoes do not protect your feet from broken glass that is frequently found in the lab Also, leather shoes protect your feet from chemical spills – canvas shoes do no

16 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Place books and personal items in the cabinet يجب ادخال الكتب والمتعلقات الشخصية فى المكان المخصص 15 Only laboratory manuals are permitted into the working area يسمح فقط بمذكرات العملى والكشكول فى منطقة العمل

17 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Listen to your instructor carefully and stand in your proper position in the lab. استمع لتعليمات مشرفك بحرص وقف دائما في مكانك المخصص 16

18 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Eating and drinking are not allowed in the lab. الاكل او الشرب غير مسموح به فى المعمل Put all food and drinks in your bag.

19 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Do not sit on lab benches. لا تجلس على أثاث المعمل 18

20 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department side talks & the mobile are not allowed in the lab. لا تتحدث مع زملائك في المعمل او في المحمول. In a laboratory setting no pushing; no shoving. Avoid Horseplay

21 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department At the end of the lab period: Exit the lab in an orderly manner. Again: no running, no pushing, no shoving. 20

22 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Read the instruction guide ahead of performing the experiment. اقرأ دليل التعليمات قبل اجراء التجربة. 21

23 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department General work procedure 22

24 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department When in doubt – ASK!!! عند الشك ---- اســــــــأ ل Do not carry out a new or unfamiliar procedure until you have been fully trained & understand the precautions necessary for safe working DO NOT GUESS!!!! DO NOT GUESS!!!!

25 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department You should wear approved safety goggles or safety glasses when instructed in the lab. يجب ارتداء نظارات الوقاية بالمعمل اذا كلفت بذلك 24 Personal Protective Equipment

26 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department This is not acceptable!!! 25

27 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Wear gloves if you will come in contact with blood specimens عينة من الدم ارتد القفاز عندما ملامسة For even more dangerous liquids, thick gloves should be used. 26

28 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Use only clean & dry glassware and never use broken or chipped glassware. استعمل فقط زجاجيات نظيفه جافه وسليمه 27 Check glassware for stars or cracks.

29 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Use only materials and equipments authorized by the instructor استعمال اى مواد او اجهزة لا يكون الا باذن المشرف المسئول 28 Be aware if the chemicals used are hazardous كن حذرا مع الكيماويات الخطيرة ان استعملت

30 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Pour from large containers to smaller ones. 29 Always ADD ACID to water Not the reverse !! Do not spit into acid! a good phrase to remember this rule. acid water

31 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Use the flame with extreme caution and Keep your head and clothing away from the flame استعمل اللهب بحرص شديد واجعل راسك وملابسك بعيدا عن اللهب 30

32 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Never carry flammable or hot and dangerous equipment or chemicals through the lab. لا تتحرك ابدا بالادوات اوالكيماويات الساخنة و الضارة اوالقابلة للاشتعال داخل المعمل 31 Handle hot glassware with holder or tongs.

33 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Always point test tubes away from yourself and others when heating وجه دائما انابيب الاختبار بعيدا عن نفسك وعن غيرك اثناء التسخين 32

34 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Never taste anything or touch chemicals with hand لا تتذوق ابدا الكيماويات او تلامسها باليد 33

35 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Test for odor of chemicals only by waving your hand above the container & sniffing cautiously from a distance اختبار الرائحة للكيماويات يكون فقط عن طريق تحريك اليد من اعلى المصدر مع الاستشناق بحذر من على بعد 34

36 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Never pour used reagents back into the bottle لاترد الكيماويات المستعملة مرة اخرى الى مصدرها 35

37 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Keep lids on bottles and containers when not in use. قم بتغطيه الزجاجيات في حاله عدم استخدامها 36

38 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Use a rubber suction bulb or other device to fill a pipette should be used whenever available 37

39 Safety Rules 38

40 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Inform the instructor immediately about any non properly working equipment or any difficulty عليك ابلاغ مشرف المعمل عند حدوث عطل في اي جهاز اي وجود اي صعوبة 39

41 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Hygiene practice 40

42 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Keep hands away from face, eyes and clothes during lab عدم ملامسة اليد لأى من الوجه والعينين و الملابس اثناء العملى 41

43 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Keep tabletops clean. Return all equipment to its original location before leaving the lab. Clean all spills immediately. 42 Always clean the instruments & laboratory area before leaving دائما نظف أدواتك ومنطقة عملك قبل مغادره المعمل

44 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Broken Glass Sweep it up right away. Always clean the instruments دائما نظف أدواتك

45 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Know how to dispose of waste Dispose all waste materials according to your instructor order. ? ? ? ? 44

46 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Wash hands with soap and water before leaving the lab. اغسل يديك بالماء والصابون قبل مغادره المعمل 45

47 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Emergency procedure 46

48 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 47 Emergency procedures includes: 1-First aid procedures are available. First aid form and safety materials are included (Appendix 1). 2- Inform the supervisor immediately about any problem or accident in the lab. An accident report form is available in the main lab office (Appendix 2). 3- If any accident happened, as burn from an acid or alkali, wash the affected area immediately with plenty of running water.

49 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department In case of accident as an acid or alkali burns Wash the affected area immediately with plenty of running water لو وقع اى حادث كحرق من حمض او قلوى اغسل المنطقة المصابة فى الحال بكثير من الماء الجارى 48

50 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Inform the supervisor immediately about any problem or accident in the lab ابلغ المشرف فورا عند حدوث اى مشكلة او حادث بالمعمل 49

51 PLATFORMPLATFORM PLATFORMPLATFORM Preparation room EXIT Lab benches IReport Room Chemical &Reagents Area Lab Safety & First Aid Biochemistry and Molecular biology Department Lab Diagram Instructor PLATFORMPLATFORM PLATFORMPLATFORM

52 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 51

53 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department 52

54 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department Know the locations and operating instructions for Safety equipments 53 Fire extinguishers Fire blanket first aid kit eye washes

55 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department First Aid Injury: Burns What To Do: Immediately flush with cold water until burning sensation is lessened. 54

56 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department First Aid Fainting: To Do: Provide fresh air and keep the head lower than the rest of the body. 55

57 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department First Aid Eye Injury: Eyes What To Do: Flush eyes immediately with plenty of water for at least 15 minutes. If a foreign object is lodged in the eye, do not allow the eye to be rubbed. 56

58 Ain Shams University/ Faculty of Medicine Medical Biochemistry & Molecular Biology Department First Aid Skin Injury: Spills on the skin What To Do: Wash with large quantities of water. For acid spills, apply baking soda solution. For base spills, apply vinegar or boric acid. 57

59 Have a Safe Year! 58


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