Presentation is loading. Please wait.

Presentation is loading. Please wait.

Modifications to an Fcγ-Fcɛ fusion protein alter its effectiveness in the inhibition of FcɛRI-mediated functions  Lisa Chan Allen, PhD, Christopher L.

Published byΒαρβάρα Πρωτονοτάριος Modified over 5 years ago

Similar presentations

Presentation on theme: "Modifications to an Fcγ-Fcɛ fusion protein alter its effectiveness in the inhibition of FcɛRI-mediated functions  Lisa Chan Allen, PhD, Christopher L."— Presentation transcript:

1 Modifications to an Fcγ-Fcɛ fusion protein alter its effectiveness in the inhibition of FcɛRI-mediated functions  Lisa Chan Allen, PhD, Christopher L. Kepley, PhD, MBA, Andrew Saxon, MD, Ke Zhang, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 120, Issue 2, Pages (August 2007) DOI: /j.jaci Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Schematic diagram of human GE proteins. The molecules are depicted with the N-terminus on the left and the C-terminus on the right. The Fcγ domains (γ hinge-Cγ2-Cγ3) are striped, the Fcε domains (Cε2-Cε3-Cε4) are dotted, the flexible linker is black, and the hemagglutinin tag is white. Amino acid substitutions are designated by their IgG1 EU index position. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Purified GE proteins visualized on SDS-PAGE. A, Two micrograms of nonreduced samples of IgE isoforms, GE proteins, and BSA were run on a 5% phosphate gel. B, One microgram reduced samples of GE proteins and BSA were run on a 12% Tris-glycine gel. Molecular weight (MW) is indicated in kilodaltons on the right. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 GE proteins and IgE bind a basophil-like cell line, Ku812. Cells were incubated with several concentrations of IgE or GE proteins, and protein binding was measured as the percentage of α-ε fluorescein isothiocyanate–positive cells detected by means of flow cytometry. Error bars represent the SD among 3 repetitions. The GE H mutant at 5 μmol/L had 1 measurement. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Effect of GE proteins on human basophil FcεRI-mediated degranulation. A, Basophils from a representative donor presensitized with 10 μg/mL anti-NP IgE, treated with 10 μg/mL protein, and activated with NP-BSA. Percentage of degranulation is taken from duplicate samples. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 GE proteins block PCA: A, GE2 versus E2G; B, GE2 versus GE S mutant; C, GE2 versus GE H mutant; and D, size exclusion chromatography-purified E2G monomers versus E2G. Mice were injected with serum from a patient with cat allergy or purified IgE plus GE proteins and then challenged after 4 hours with Fel d 1 in Evans Blue dye. ∗Serum with 5 μg/mL myeloma IgE. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Modifications to an Fcγ-Fcɛ fusion protein alter its effectiveness in the inhibition of FcɛRI-mediated functions  Lisa Chan Allen, PhD, Christopher L."

Similar presentations

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,
Shea butter contains no IgE-binding soluble proteins

Shea butter contains no IgE-binding soluble proteins
Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M

Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M
Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease  Merja T. Augustin, MD, Jorma Kokkonen, MD,
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD 
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Fatty acids, inflammation, and asthma

Fatty acids, inflammation, and asthma
Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M

Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M
Christopher L. Kepley, PhD, Bridget S. Wilson, PhD, Janet M

Christopher L. Kepley, PhD, Bridget S. Wilson, PhD, Janet M
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Accelerated dissociation of IgE-FcεRI complexes by disruptive inhibitors actively desensitizes allergic effector cells  Alexander Eggel, PhD, Günther.

Accelerated dissociation of IgE-FcεRI complexes by disruptive inhibitors actively desensitizes allergic effector cells  Alexander Eggel, PhD, Günther.
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Molecular engineering of a therapeutic antibody for Blo t 5–induced allergic asthma  J.H. Sherlynn Chan, BSc(Hons), Yen Leong Chua, MSc, Hong Yong Peh,

Molecular engineering of a therapeutic antibody for Blo t 5–induced allergic asthma  J.H. Sherlynn Chan, BSc(Hons), Yen Leong Chua, MSc, Hong Yong Peh,
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Fatty acids, inflammation, and asthma

Fatty acids, inflammation, and asthma
Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers 

Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers 
A mouse Fcγ-Fcε protein that inhibits mast cells through activation of FcγRIIB, SH2 domain–containing inositol phosphatase 1, and SH2 domain–containing.

A mouse Fcγ-Fcε protein that inhibits mast cells through activation of FcγRIIB, SH2 domain–containing inositol phosphatase 1, and SH2 domain–containing.
In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing  Nikolaos Douladiris, MD,

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing  Nikolaos Douladiris, MD,
Molecular determinants of allergen-induced effector cell degranulation

Molecular determinants of allergen-induced effector cell degranulation

Similar presentations

Ads by Google