1. Volume 67, Issue 4, Pages 716-726 (April 2015)
Novel Therapeutic Approach for Neurogenic Erectile Dysfunction: Effect of Neurotrophic Tyrosine Kinase Receptor Type 1 Monoclonal Antibody 
Guiting Lin, Huixi Li, Xiaoyu Zhang, Jianwen Wang, Uwais Zaid, Melissa T. Sanford, Victor Tu, Alex Wu, Lin Wang, Fei Tian, Helen Kotanides, Venkatesh Krishnan, Guifang Wang, Hongxiu Ning, Lia Banie, Ching-Shwun Lin, Gary G. Deng, Tom F. Lue 
European Urology 
Volume 67, Issue 4, Pages (April 2015)
DOI: /j.eururo
Copyright © 2014 European Association of Urology Terms and Conditions

2. Fig. 1
Intracavernous pressure (ICP) during cavernous nerve (CN) electrostimulation at 6 wk after surgery. (A–G) Representative ICP recordings for the experimental groups defined in Table 1. The colored bar denotes the 50-s CN electrical stimulation. (H) Effect of TrkA-mAb treatment on the ratio of ICP to mean arterial pressure (MAP). Bars denote the mean ICP/MAP ratio (± standard error of the mean). * p<0.05 compared with the vehicle group.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

3. Fig. 2
Sexual behavior test. (A) Proportion of rats exhibiting chasing (C), mounting (M), intromission (I), or ejaculation (E) behaviors in the three groups. Bars denote the number of rats exhibiting a particular activity. (B–E) Average number of (B) chasing, (C) mounting, (D) intromission, and (E) ejaculation behaviors exhibited in the three groups. Bars denote the average number of particular activities recorded for one rat in each group. Data are presented as mean ± standard error of the mean.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

4. Fig. 3
Tyrosine hydroxylase (TH) and neuronal nitric oxide synthase (nNOS) expression in cavernosum. (A) Representative images of western blots for TH and nNOS in cavernosum for the experimental groups defined in Table 1. (B) Effect of neurotrophic tyrosine kinase receptor type 1 monoclonal antibody (TrkA-mAb) treatment on the TH/nNOS ratio following cavernous nerve injury. The band density was semiquantitatively determined using Image J. Bars denote the mean TH/nNOS ratio (± standard error of the mean) * p<0.05 compared with the vehicle group. (C) The black rectangle denotes the area of the corpus cavernosum selected for comparison. (D) Representative immunofluorescence staining of TH (red) and nNOS (green) in two consecutive penile midshaft specimens. Original magnification, ×400. DAPI=4′,6-diamidino-2-phenylindole.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

5. Fig. 4
Neuronal nitric oxide synthase (nNOS) staining of dorsal nerves. (A) Representative images of nNOS-positive fibers (green) and neurofilament (NF, red) in the penile dorsal nerve for the experimental groups defined in Table 1. Original magnification, ×400. (B) Effect of neurotrophic tyrosine kinase receptor type 1 monoclonal antibody (TrkA-mAb) treatment on nNOS-positive fibers in the penile dorsal nerve. DAPI=4′,6-diamidino-2-phenylindole; HPF=high-power field. * p<0.05 compared with the vehicle group.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

6. Fig. 5
Phalloidin staining of penile midshaft specimens. (A) Representative images of smooth muscle stained with Alexa-594–conjugated phalloidin (red) for the experimental groups defined in Table 1. Original magnification, ×40 and ×200. The white rectangle denotes the area selected for amplification. (B) Representative image of western blot for smooth muscle actin (SMA) in cavernosum. (C) Effect of neurotrophic tyrosine kinase receptor type 1 monoclonal antibody (TrkA-mAb) treatment on SMA content following cavernous nerve injury. The density was determined semiquantitatively using Image J. Bars denote the mean SMA/β-actin ratio (± standard error of the mean). DAPI=4′,6-diamidino-2-phenylindole. *p<0.05 compared with the vehicle group.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

7. Fig. 6
Masson trichrome staining of penile midshaft specimens. (A) Representative images of actin and collagen staining for the experimental groups defined in Table 1. Smooth muscle and connective tissue in the corpus cavernosum are stained red and green, respectively. Original magnification, ×40 and ×200. (B) Effect of neurotrophic tyrosine kinase receptor type 1 monoclonal antibody (TrkA-mAb) treatment on the ratio of smooth muscle to collagen in the corpus cavernosum. Bars denote the mean densitometry ratio between smooth muscle content and collagen content per field (± standard error of the mean). * p<0.05 compared with the vehicle group.
European Urology  , DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions