1. Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells.
Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Quantitative proteomics of GFP immunoprecipitations from HEK293 cells and primary rat neurons transfected or transduced with GFP, GFP-(GR)149, or (PR)175-GFP. (A, B) Volcano plots of interactomes from neurons (A) and HEK293 cells (B). The data for all proteins are plotted as log2-fold change versus the −log10 of the P-value. Gray line indicates significance cutoff. Enriched protein families are color coded: cytoplasmic ribosome (blue), mitochondrial ribosome (red), stress granules (brown) (Jain et al, 2016), and methylosome (green). The top enriched proteins (sorted by fold-change) and the proteins analyzed in this study are labeled with gene names. Filled circles indicated that the protein was significantly altered in the other cell type.
Hannelore Hartmann et al. LSA 2018;1:e
© 2018 Hartmann et al.