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WWW.BIOMED.DREXEL.EDU/ResearchPortfolio/ School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Kambiz Pourrezaei, Ph.D. Drexel University.

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Presentation on theme: "WWW.BIOMED.DREXEL.EDU/ResearchPortfolio/ School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Kambiz Pourrezaei, Ph.D. Drexel University."— Presentation transcript:

1 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Kambiz Pourrezaei, Ph.D. Drexel University School of Biomedical Engineering, Science & Health Systems NANO-OPTICS FOR CELLULAR INVESTIGATION Focus on Microscopy 2004 University City Sheraton Philadelphia, Pennsylvania

2 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] OVERVIEW Optical Nanoprobes in NSOM/Raman: Membrane and Intracellular Detection Mark Contarino, Ed Keough, Irwin Chaiken, Som Tyagi, Kambiz Pourrezaei Surfaced Enhanced Raman Spectroscopy: Label-Free Detection using SERS Optimized Nanoprobes Vishal Kamat, Ed Keough, Mark Contarino, Elisabeth Papazoglou, Kambiz Pourrezaei, Som Tyagi Quantum Dots: Biological Applications of Fluorescent Nanoparticles Bahar Edrissi, Amir Rezvan, Mark Contarino, Johan Verjans, Chris Reutelingsperger, Jagat Narula, Som Tyagi, Elisabeth Papazoglou, Peter Lelkes

3 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Fiber Optic Nano-probe Fabrication By exposing the silica core, the fiber is pulled into nano probes using the P2000 Micropipette Puller. Fiber tips are typically 30-60nm in diameter. Fibers are cleaned using a wet chemical dip-process. By varying the layering method and deposition parameters, we can have smooth & uniform gold film or uniform gold blobs on the surface of these nano probes. P2000 Micropipette Puller 30nm Au Sputtered Using Standard Smooth Layering E-Beam Evaporation of 100nm Au

4 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Nanonics/Renishaw combination SPM/Raman system Simultaneous AFM/NSOM Topographic Information Nanoprobe Dimensions Allow for Sub- Wavelength (< 100 nm) Investigations Evanescent Illumination Reduces Background Fluorescence System Provides Controlled Probe Insertion

5 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Φ = 500 nm λ Excitation = 488nm λ Emission = 518nm NSOM Fluorescence Imaging Schematic of monochromatic light delivered through tapered nanoprobe aperture that excites FITC (green) labelled IgG (red) physi-adsorbed on glass slide. [concentration = 50ug/ml]

6 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Topographic Data Simultaneous collection

7 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Fluorescence Recovery Wavelength (nm) Counts Fluorescence collected of FITC-labeled anti-5-His IgG using Nanonics/ Renishaw platform under 488nm excitation through metallic coated nanoprobe (500nm aperture)

8 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Intracellular Targets Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Labeling procedure conjugates only exposed silica aperture, capturing intracellular fluorescent targets. membrane

9 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Antibody Functionalization of Silica Surface Phase contrast and fluorescent contrast images of control fiber (left) and FITC labeled antibody functionalized fiber (right). The fluorescence in the control is due to a defect in the fiber, as seen in the phase image.

10 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Cell under inverted microscope Collect/Count Photons Quantify Concentration UV light needle-like fiber Y <150 nm Cullum and Vo-Dihn, 2000

11 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Preliminary Cell Viability Trypan blue experiments with four probe diameters. After several cells were probed with the same tip, there was an observed residue buildup on the tip. After this event, subsequent probes resulted in membrane adherence to the probe, rupturing cellular integrity

12 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] SERS Surface Enhanced Raman Spectroscopy Metallic clusters of < 200nm have shown to enhance the normally weak Raman scattering by as much as ! Apertureless probes could bring a Raman signal inside cells, fingerprinting biomolecules with a unique Raman spectra

13 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] SERS Optimized Nano-Probes The tapered probe is coated with gold film with the fabrication of well organized gold features on the surface of the tip (shown above). The tip of the probe will give optimum condition for maximum enhancement of the Raman signal. Functionalization of the tip with target specific antibody will enable us to detect any antigen in the cell.

14 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] SERS for ZnO RED: Enhanced Raman signal from ZnO (435 cm-1) on the gold coated probe. BLUE: Standard Raman signal for ZnO Observed enhancement is around 100xs

15 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] SERS for Collagen - Type I In the inset : Red Enhanced Raman Spectrum of Collagen Type I. Violet Normal Raman Spectrum of Collagen Type I The enhancement factor is around 50xs. Amine Peak

16 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Future Goals & Achievements oFabrication of nm Gold beads at the tip of these nano probes to have Tip Enhanced Raman Spectroscopy (TERS). oTERS for single cell analysis. oAnalysis of Protein Structure & its folding process using TERS. oDetermination of intracellular signaling pathways using TERS. oIntegrating Near Field Scanning Optical Microscope (NSOM) & TERS for cell analysis with 50nm resolution.

17 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Elisabeth S. Papazoglou, Bahar Edrissi, Amir Rezvan, Mark Contarino, Johan Verjans, Chris Reutelingsperger, Jagat Narula, Kambiz Pourrezaei, Peter Lelkes Q – Dots Biological Applications of Fluorescent Nanoparticles

18 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Continuous Absorption Profile Narrow Gaussian-like Emission Profile Highly Luminescent with Single Excitation Source Unique Optical Properties

19 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] In vivo imaging of cardiac tissue apoptosis using Annexin-V targeted quantum dots. -Ischemia/reperfusion studies in live animal murine model in collaboration from University Maastricht, Neth. Q-dot uptake in liver Aggregation in cardiac vasculature Q-dots in lungs

20 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Instrumental Setup

21 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327]

22 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Q-Dot – Cell Interactions Case I: Absence of specific Targeting Functionality Collagen Matrix Remodeling promotes Q-dot Uptake BAEC (Bovine Aortic Endothelial Cells) + CT (48 hrs) – Transferred Cells Gelatin Coated 8-well slide Phase Contrast – 40X BAEC + CT (48 hrs) – Transferred Cells Gelatin Coated 8-well slide Fluorescence – FITC Filter – 40X – 1s Exposure time

23 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Q-Dot – Cell Interactions Case I: Absence of specific Targeting Functionality Matrix-remodeled Uptake in Carcinoma Cells HDC (Human Ductile Carcinoma) + Lake Placid Blue-Carboxyl Terminated (CT) – Transferred Cells Gelatin Coated 8-well slide Phase Contrast – 40X HDC + CT (48 hrs) – Transferred Cells Gelatin Coated 8-well slide Fluorescence – FITC Filter – 40X – 1s Exposure Time

24 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Q-Dot – Cell Interactions Case II: Specific Targeting Functionality QD:EGF, through STV-Biotin A431 (Human Squamous Carcinoma) + previously mixed 6:1 molar ratio of Streptavidin-Qdots (655 tracker) and Biotin-EGF – 5 min Phase Contrast – 10X A431 (Human Squamous Carcinoma) + previously mixed 6:1 molar ratio of Streptavidin-Qdots (655 tracker) and Biotin-EGF – 5 min Fluorescence – 10X – TRITC – 1s Exposure Time

25 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Q-Dot – Cell Interactions Case II: Specific Targeting Functionality – Our Data Dynamic Observation after Addition of 6:1 Molar Ratio of QD:EGF A431 (Human Squamous Carcinoma) + previously mixed 6:1 molar ratio of Streptavidin-Qdots (655 tracker) and Biotin-EGF – 40 min Phase Contrast – 10X A431 (Human Squamous Carcinoma) + previously mixed 6:1 molar ratio of Streptavidin-Qdots (655 tracker) and Biotin-EGF – 40 min Fluorescence – 10X – TRITC – 1s Exposure Time

26 School of Biomedical Engineering, Science & Health Systems V 1.0 SD [020327] Q-Dots – Our Goals I) Study internalization of EGF receptor in Squamous Cell Carcinoma Ligand mediated Anti-body mediated (TJU- Dr. U. Rodeck) II) Study IL-15 mechanism (Interleukin expressed in asthma) Protein Institute (Dr. I. Chaiken) III) Combine with our Nanoprobes


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