3Parts of ScopeOcular (eyepiece) lens – closest to eye; 10X standard magnificationHead – ocular lens insertedArm – hold while carryingMechanical stage – has window over which specimen is placed; holds down slide; knobs for moving slide back/forth, up/downIris diaphragm – allows more or less light through specimen; acts like iris of eyeLight knob – adjusts amount of lightLight source – electrical lightCourse Adjustment knob – moves stage up and down to focus on specimenFine Adjustment knob – minutely moves stage up and down for fine tuning or precise focusingBase – stand of scopeAbbey Condenser – small lens below window of stage varies light; do NOT touch!Nosepiece – objectives attached; rotates objectivesObjective lenses – 4X, 10X, 40X, 100X; lenses closest to object/specimen
4Objective Lenses 4X – Scanning power; scans all of specimen 10X - Low power; see more detail than scanning but less of specimen40X – High power; see much greater detail than low power but very little of specimen100X – oil immersion lens; used with special oil; used in Microbiology
5Total Magnification Ocular Lens X Objective lens How much specimen is totally magnifidedi.e. 10X x 40X = 400X total magnification – therefore the specimen is 400 times larger than originally
6Focusing/Viewing Specimen ALWAYS begin with 4X scanning power to position specimen properly in windowFocus with course adjustment knob; fine tune with fine adjustment knobCarefully turn nose piece so that 10X is over specimen/window of stage.VERY carefully turn nose piece so that 40X is over specimen/window of stage.Focus with FINE ADJUSTMENT KNOB ONLYCourse adjustment knob outer; fine adjustment knob innerAdjust light with light knob or iris lever accordingly
7TransportingALWAYS carry the microscope with BOTH hands; one under base, one holding armAlways return microscope to proper numbered slot in scope cabinet
8Returning Scope Before returning the scope to the cabinet--- Clean all lensesRemove slide from stageTurn course focusing knob so that stage is at lowest pointRotate nose piece so that 4X objective over windowAccordian the power cord and secure with velcro strip
9TermsParfocal – once a specimen is in focus on lower power then switching to next higher power, specimen should remain in focusField of View – circle of light seen when looking through ocular lens.Working Distance – distance between bottom of objective lens and specimenDepth of Field – depth of specimen
10Calming FrustrationsBefore using the microscope, ALWAYS clean the stage window, light source and most importantly ALL lenses and the slide.This will save tons of time!