1. The Role of MGMT Testing in Clinical Practice
Milena Cankovic, Marina N. Nikiforova, Matija Snuderl, Adekunle M. Adesina, Neal Lindeman, Patrick Y. Wen, Eudocia Q. Lee 
The Journal of Molecular Diagnostics 
Volume 15, Issue 5, Pages (September 2013)
DOI: /j.jmoldx
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Schematic of the MGMT promoter region with CpG island and the CpGs most commonly interrogated by MSP, MethyLight qMSP, and pyrosequencing.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Detection of MGMT methylation by MSP and MethyLight qMSP. Isolated DNA from tumor specimen is treated with sodium bisulfite, which converts unmethylated cytosine (C) into uracil (U) but leaves methylated cytosine (mC) unchanged. The modified DNA is used as a template for MSP or MethyLight qMSP. MSP/agarose gel electrophoresis demonstrates absence of MGMT methylation in tumor 1 (only the unmethylated band, U, is visualized on the gel) and presence of methylation in tumor 2 (a strong methylated band, M, is visualized). MethyLight qMSP shows absence of MGMT methylation in tumor 1 (amplification is detected only in the housekeeping gene COL2A1 control) and presence of MGMT methylation in tumor 2 (amplification is detected in both the housekeeping gene COL2A1 and MGMT).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
Detection of MGMT methylation by pyrosequencing. Pyrograms demonstrate unmethylated (<20% methylation), methylated (>80% methylation), and partially methylated (>20% and <80% of CpG islands) MGMT.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
MS-MLPA protocol. The MS-MLPA procedure can be divided into four steps: i) DNA denaturation and hybridization of MLPA probes; ii) ligation and digestion; iii) PCR and separation of amplification products by capillary electrophoresis; and iv) data analysis.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions