1. ADVANCED TECHNOLOGY IN BLOOD SEPARATION
Lmb Technologie GmbH
ADVANCED TECHNOLOGY IN BLOOD SEPARATION

2. ErySep ! Unique and New Blood Separation Technology
Blood Separation without mechanical devices
Without Centrifuge
Without Separation Press
Without Power Supply
Blood Separation by gravity force
ErySep separates whole blood into two components ( RED CELL and PLASMA ) by itself without any other instruments
Separated Blood Components in perfect quality
Cells Free PLASMA
Leukocyte Free RED CELL
Concentrated RED CELL ( 55%-60% HTC )

3. ErySep ! Unique and New Blood Separation Technology
Approved Clinical Results
Studying on different parameters such as; pH, LDH, K, P, NH3, Osmolality, 2,3-DPG, Total protein, Hemoglobin in TU (g/unit), Loss of Hb, Haemolysis, Leucocytes, F VIII activity, Platelets,
the results have been published in international scientific magazines.
Validation in different Country Blood Banks
German Blood Banks Indonesia Blood Banks
UK Blood Banks Malaysia Blood Banks
Spain Blood Banks Myanmar Blood Banks
Bulgaria Blood Banks Taiwan Blood Banks
Greece Blood Banks Philippines Blood Banks
Chech Blood Banks Egypt Blood Banks
Slovakia Blood Banks Yemen Blood Banks
Belgium Blood Banks Jordan Blood Banks
Turkish Blood Banks Saudi Blood Banks
Russia Blood Banks
All results meets with European Blood Components Standard
Guide to the preparation, use and quality assurance of blood components - 15th Edition (2009)

4. ErySep ! Unique and New Blood Separation Technology
WHY ERYSEP?
ErySep is user friendly
No necessary special education
Work in all kind of environment
(outdoor/indoor)
It is activated in 5 minute, and no need extra efforts
Staff can do their other daily work
ErySep complete process by itself within 75 minutes.
ErySep is a Closed Sterile System
It can not effect outside conditions
Cost Reduction
No centrifuge & extractor investment
No maintainance cost in routine usage
No power supply
Easy Handling
24 month shelflife in room condition
Easy Product exchange & replacement
Portable & ready work wherever needed
Ready to use any time for any amount
Expert can obtain specific demanded quantity
ErySep can answer night time demand

5. ErySep ! Unique and New Blood Separation Technology
ErySep® is a disposable system
has standard triple blood bag
whole blood bag
red cell bag
plasma bag with an integrated air drainage bag
has standard leukocyte filter
has test bag with vacu-container port
has membrane filter embedded into separation chamber unit 5 4 1 2 3 1 6 2 4 5 6 3

6. ErySep ! Unique and New Blood Separation Technology
HOW ErySep® WORKS?
Donor Blood is taken into Mother bag by using an integrated needle on the system.
ErySep works by gravity force, does not need any other instruments or power supply.
System can activate just by breaking internal plastic valves, after hanging to a infusion stand.
Tubular membrane with a diameter of <1 mm and hollows (pores) 0,2 µm make physical separation; Red Cells are pushed into Red Cell Bag, Plasma are pushed into Plasma Bag through the membrane filter.

7. ErySep ! Unique and New Blood Separation Technology
Medical ADVANTAGES of ErySep®
ErySep supplies Leuko-depleted Red Cell & Plasma
Erysep supplies high HTC level into the Packed Red Cell Bag.
ErySep supplies plasma, which is ready for FFP
ErySep supplies Packed Red Cell Bag, which can be store 42 days.
Leuko-depleted components are precious for Oncology and Hematology Patients
Make available the fresh components at hospital for surgical operation purposes.
Make available the fresh components for the patients need routine transfusion at hinterland.

8. Datas from Hospital Prag Dr. Bohaniek
Time
Day 0
Day 42
pH Average value
LDH (µkat/l) Average value
K (mmol/l) Average value
P (mmol/l) Average value
NH3 (µmol/l) Average value
Osmolality (mOsm/l) Average value
ATP (µmol/g Hb) Average value
DPG (µmol/g Hb) Average value
Hemolysis Average value
0.7205

9. Dr. med.Dr.rer.nat Kurt Mannemann-Pohl
Rating and Validation of ErySepe..aıood donation and Blood separation device
Dr. med.Dr.rer.nat Kurt Mannemann-Pohl
Germany – Hamburg - Pohl Fachaat fOr Laboratoriumsmedizin Facharzt fOr Transfusionsmedizin DipL. Chem.
BACKGROUND: ErySep Blood Donation and Blood separation device has been validated in Hamburg at Marien Krankenhaus Blood bank. Whole results have been reported as clinical study.
STUDY DESIGN AND METHODS: The used analysis methods performed in the laboratory of the Marienkrankenhaus Hamburg and are listed below. All analysers and reagents were CE certified.
All analysis methods were under permanent supervision of our quality control system with permant internal and four external controls per year.

10. RBC Results German Studies ( n=15 )
Value
Avarage volume unit
346 ml Hb
17,4 g/dl
Haematocrit
54%
Leucocytes in unit on day 1
0,03x10 6
Protein / unit
3,3 g/unit
K+ in RBC on day 1
0,28mmol/l
Free Hb on day 1
all<0,03 g/l
Free Hb on day 42
1,5 g/l
Units were sterile as tested with BacT/Alert Culture System of BioMerieux
Plasma Results German Studies ( n=15 )
Plasma volume
232 ml
Protein concentration
53,5 g/l
Leukocytes per unit
0,02 WBC/unit
Fibrinogen
191 mg/dl
ATIII
82% of normal
vWF-antigen
F VIII
Units were sterile as tested with BacT/Alert Culture System of BioMerieux

11. CONCLUSION: Easy to perform blood separation without the need for centrifuge, separator or even electricity; For donor and patient the procedure do not change; Plasma has a very good quality and is better than the standard blood bank quality; RBC’s are better compared to standard blood bank quality. Remarkable is the very low WBC contect of far less than 1 WBC/µl ( effect of the hollow fibres ); Variation of measured parameters were all within the accepted limits for blood bank routine.
The ErySep-Device is a ready to use blood donation and separation system.
It is allowing, to produce leukocyte depleted erythrocyte concentrate and a cell free plasma with an elegant and save procedure with less equipment and limited manpower.
In one of the last validations of ErySep ( ). we can say, that the results of the related labor tests are identical with the standards of the European Guidelines.
The ErySep is separating the donor blood only by earth gravitation, without electricity or using of centrifuges etc.

12. Spain / Cord Blood Bank. Banc de Sang i Teixits. Barcelona
Cord Blood separation for obtaining fetal erithrocytes.Preliminar results. / December 2009.
Marta Torrabadella
Spain / Cord Blood Bank. Banc de Sang i Teixits. Barcelona
BACKGROUND: Cord blood separation with membrane filter technology is totally new and available in 2009 commercially. The first clinical study made by Dr Brune in Germany. This is 2nd study, which was perfomed in December 2009.
STUDY DESIGN AND METHODS: The aim of the study is to show characterization of cord blood components after separation with ErySep system. ErySep system offers the possibility of blood separation of plasma and erythrocyte concentrate by gravity

13. RBC concentration Value Range Mean fetal Hb 67,8% 76- 58,6
Median Hb obtained in RBC concentrate
5,5
1,8-10,6
Percentage hemolysis
0,26%
0,13 – 0,48
LDH (U/L) : Basal (n= 3 )
143
K+ (mmol/l) : Basal (n= 3)
1,63
1,54 - 1,76
Plasma
Value
Range
Plasma Volume (ml) (n= 11)
31.36
5 - 85
Platelet concentration (CellDyn) in Plasma (n= 13)
77.30
Protein (g/L) in Plasma (n= 12)
2,45
2- 3,3
K+ mmol/l in Plasma (n= 7)
4,38
3,13- 5,96
LDH (UI) in Plasma (n= 7)
370,85
Free Hb (n= 12)
0.022
0,00- 0,06
WBC/µL (n= 13)
0.076
0- 1