Presentation on theme: "ADVANCED TECHNOLOGY IN BLOOD SEPARATION"— Presentation transcript:
1 ADVANCED TECHNOLOGY IN BLOOD SEPARATION Lmb Technologie GmbHADVANCED TECHNOLOGY IN BLOOD SEPARATION
2 ErySep ! Unique and New Blood Separation Technology Blood Separation without mechanical devicesWithout CentrifugeWithout Separation PressWithout Power SupplyBlood Separation by gravity forceErySep separates whole blood into two components ( RED CELL and PLASMA ) by itself without any other instrumentsSeparated Blood Components in perfect qualityCells Free PLASMALeukocyte Free RED CELLConcentrated RED CELL ( 55%-60% HTC )
3 ErySep ! Unique and New Blood Separation Technology Approved Clinical ResultsStudying on different parameters such as; pH, LDH, K, P, NH3, Osmolality, 2,3-DPG, Total protein, Hemoglobin in TU (g/unit), Loss of Hb, Haemolysis, Leucocytes, F VIII activity, Platelets,the results have been published in international scientific magazines.Validation in different Country Blood BanksGerman Blood Banks Indonesia Blood BanksUK Blood Banks Malaysia Blood BanksSpain Blood Banks Myanmar Blood BanksBulgaria Blood Banks Taiwan Blood BanksGreece Blood Banks Philippines Blood BanksChech Blood Banks Egypt Blood BanksSlovakia Blood Banks Yemen Blood BanksBelgium Blood Banks Jordan Blood BanksTurkish Blood Banks Saudi Blood BanksRussia Blood BanksAll results meets with European Blood Components StandardGuide to the preparation, use and quality assurance of blood components - 15th Edition (2009)
4 ErySep ! Unique and New Blood Separation Technology WHY ERYSEP?ErySep is user friendlyNo necessary special educationWork in all kind of environment(outdoor/indoor)It is activated in 5 minute, and no need extra effortsStaff can do their other daily workErySep complete process by itself within 75 minutes.ErySep is a Closed Sterile SystemIt can not effect outside conditionsCost ReductionNo centrifuge & extractor investmentNo maintainance cost in routine usageNo power supplyEasy Handling24 month shelflife in room conditionEasy Product exchange & replacementPortable & ready work wherever neededReady to use any time for any amountExpert can obtain specific demanded quantityErySep can answer night time demand
5 ErySep ! Unique and New Blood Separation Technology ErySep® is a disposable systemhas standard triple blood bagwhole blood bagred cell bagplasma bag with an integrated air drainage baghas standard leukocyte filterhas test bag with vacu-container porthas membrane filter embedded into separation chamber unit541231624563
6 ErySep ! Unique and New Blood Separation Technology HOW ErySep® WORKS?Donor Blood is taken into Mother bag by using an integrated needle on the system.ErySep works by gravity force, does not need any other instruments or power supply.System can activate just by breaking internal plastic valves, after hanging to a infusion stand.Tubular membrane with a diameter of <1 mm and hollows (pores) 0,2 µm make physical separation; Red Cells are pushed into Red Cell Bag, Plasma are pushed into Plasma Bag through the membrane filter.
7 ErySep ! Unique and New Blood Separation Technology Medical ADVANTAGES of ErySep®ErySep supplies Leuko-depleted Red Cell & PlasmaErysep supplies high HTC level into the Packed Red Cell Bag.ErySep supplies plasma, which is ready for FFPErySep supplies Packed Red Cell Bag, which can be store 42 days.Leuko-depleted components are precious for Oncology and Hematology PatientsMake available the fresh components at hospital for surgical operation purposes.Make available the fresh components for the patients need routine transfusion at hinterland.
8 Datas from Hospital Prag Dr. Bohaniek TimeDay 0Day 42pH Average valueLDH (µkat/l) Average valueK (mmol/l) Average valueP (mmol/l) Average valueNH3 (µmol/l) Average valueOsmolality (mOsm/l) Average valueATP (µmol/g Hb) Average valueDPG (µmol/g Hb) Average valueHemolysis Average value0.7205
9 Dr. med.Dr.rer.nat Kurt Mannemann-Pohl Rating and Validation of ErySepe..aıood donation and Blood separation deviceDr. med.Dr.rer.nat Kurt Mannemann-PohlGermany – Hamburg - Pohl Fachaat fOr Laboratoriumsmedizin Facharzt fOr Transfusionsmedizin DipL. Chem.BACKGROUND: ErySep Blood Donation and Blood separation device has been validated in Hamburg at Marien Krankenhaus Blood bank. Whole results have been reported as clinical study.STUDY DESIGN AND METHODS: The used analysis methods performed in the laboratory of the Marienkrankenhaus Hamburg and are listed below. All analysers and reagents were CE certified.All analysis methods were under permanent supervision of our quality control system with permant internal and four external controls per year.
10 RBC Results German Studies ( n=15 ) ValueAvarage volume unit346 mlHb17,4 g/dlHaematocrit54%Leucocytes in unit on day 10,03x10 6Protein / unit3,3 g/unitK+ in RBC on day 10,28mmol/lFree Hb on day 1all<0,03 g/lFree Hb on day 421,5 g/lUnits were sterile as tested with BacT/Alert Culture System of BioMerieuxPlasma Results German Studies ( n=15 )Plasma volume232 mlProtein concentration53,5 g/lLeukocytes per unit0,02 WBC/unitFibrinogen191 mg/dlATIII82% of normalvWF-antigenF VIIIUnits were sterile as tested with BacT/Alert Culture System of BioMerieux
11 CONCLUSION: Easy to perform blood separation without the need for centrifuge, separator or even electricity; For donor and patient the procedure do not change; Plasma has a very good quality and is better than the standard blood bank quality; RBC’s are better compared to standard blood bank quality. Remarkable is the very low WBC contect of far less than 1 WBC/µl ( effect of the hollow fibres ); Variation of measured parameters were all within the accepted limits for blood bank routine.The ErySep-Device is a ready to use blood donation and separation system.It is allowing, to produce leukocyte depleted erythrocyte concentrate and a cell free plasma with an elegant and save procedure with less equipment and limited manpower.In one of the last validations of ErySep ( ). we can say, that the results of the related labor tests are identical with the standards of the European Guidelines.The ErySep is separating the donor blood only by earth gravitation, without electricity or using of centrifuges etc.
12 Spain / Cord Blood Bank. Banc de Sang i Teixits. Barcelona Cord Blood separation for obtaining fetal erithrocytes.Preliminar results. / December 2009.Marta TorrabadellaSpain / Cord Blood Bank. Banc de Sang i Teixits. BarcelonaBACKGROUND: Cord blood separation with membrane filter technology is totally new and available in 2009 commercially. The first clinical study made by Dr Brune in Germany. This is 2nd study, which was perfomed in December 2009.STUDY DESIGN AND METHODS: The aim of the study is to show characterization of cord blood components after separation with ErySep system. ErySep system offers the possibility of blood separation of plasma and erythrocyte concentrate by gravity
13 RBC concentration Value Range Mean fetal Hb 67,8% 76- 58,6 Median Hb obtained in RBC concentrate5,51,8-10,6Percentage hemolysis0,26%0,13 – 0,48LDH (U/L) : Basal (n= 3 )143K+ (mmol/l) : Basal (n= 3)1,631,54 - 1,76PlasmaValueRangePlasma Volume (ml) (n= 11)31.365 - 85Platelet concentration (CellDyn) in Plasma (n= 13)77.30Protein (g/L) in Plasma (n= 12)2,452- 3,3K+ mmol/l in Plasma (n= 7)4,383,13- 5,96LDH (UI) in Plasma (n= 7)370,85Free Hb (n= 12)0.0220,00- 0,06WBC/µL (n= 13)0.0760- 1