9 LAB WORK Make Competent Cells using JM109 Test our Competent Cells Get Familiarized with ProtocolsNew England Bio LabsOpen Wet WareReagents and buffer preparationTransformationMini PrepElectrophoresisDigestionLigation
10 EXPERIMENT DESIGN OBJECTIVES Test operation using 40mM KNO₃(if needed) under different:Substrates:LBMinimal essential mediumSaline solutionTemperatures:8°C23°C37°CImprove the characterization of pre-existing BioBricks
16 CONCLUSIONDetermining the experimental conditions for optimal results for The Renbo.Best growth inImproving characterization of pre-existing BioBricksNew results for BBa_K and BBa_k in different substrates and temperatures.The PyeaR promoter showed a better activity over the HybB promoter.
19 PROBLEMSHow to introduce Synthetic Biology into our society.Be able to find all the availability of institutions and the clear regulatory frame to develop our project.OBJECTIVESThe development of an outreach campaign about synthetic biology basic topics for every kind of public.The development of a interactive software introducing the basics of synthetic biology and the iGEM competition.The development of a guidance software that assists the user in the execution of projects related with synthetic biology.
20 OUTREACHSynthetic Biology Tour with brochures, visuals, speeches and surveys.
23 Our team is also part of a new project called “Community Bricks”, a post iGEM group, that will be released this year. We are also participating in the set up of the Alumni iGEM.
24 This software is available on Visual Basic 2010. SB UTP 1.0SB UTP 1.0 is an interactive and educational program that introduces Synthetic Biology and iGEM to the users.This software is available on Visual Basic 2010.
30 This software is available. At the end of every question of the program, you will find a website or a paper as reference about the topic.Our future objective for this software is that it will end as a data base, so that teams of any part of the world could use it and also add more information.We exhort all of the future teams to complement this software.This software is available.