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PROJECT OBJECTIVES & OVERVIEW

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Presentation on theme: "PROJECT OBJECTIVES & OVERVIEW"— Presentation transcript:

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2 PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS HUMAN PRACTICE PROJECT

3 UTP-PANAMA TEAM 2011 INSTRUCTOR & DIRECTOR UNDERGRADUATE STUDENTS:
Grimaldo Elías Ureña M. HUMAN PRACTICE ADVISOR Lucia L. Palma UNDERGRADUATE STUDENTS: Emmanuel Garuz Fotis Stringos Alicia Racine Miguel Cardenas Salomon Mitre Valery Diaz Naicolette A. Muñoz Jose Ng Arturo Nieves Dukakis Tejada Kesia Barrows Michelle Camarena

4 SYNBIO ENGINEERING TOOL KIT
Engineering tools obtained from biological resources Agricultural, Food & Civil-Environmental Engineering Mechanical and Material Sciences Engineering Electrical & Computational Engineering

5 TEAM MAIN OBJECTIVES Design of powerful sensors
Improvement of organisms ability to survive under temperature changes Focus on environmental and climatic issues. Applications

6 PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS HUMAN PRACTICE PROJECT

7 “THE RENBO” (THERMOGENIC RESPONSE NUTRIENT BIOSENSOR)
Nitrates biosensor (BioBrick BBa_K381001) AOX periplasmatic heat generator (BioBrick BBa_K410000)

8 PROJECT DESCRIPTION

9 LAB WORK Make Competent Cells using JM109 Test our Competent Cells
Get Familiarized with Protocols New England Bio Labs Open Wet Ware Reagents and buffer preparation Transformation Mini Prep Electrophoresis Digestion Ligation

10 EXPERIMENT DESIGN OBJECTIVES
Test operation using 40mM KNO₃(if needed) under different: Substrates: LB Minimal essential medium Saline solution Temperatures: 8°C 23°C 37°C Improve the characterization of pre-existing BioBricks

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16 CONCLUSION Determining the experimental conditions for optimal results for The Renbo. Best growth in Improving characterization of pre-existing BioBricks New results for BBa_K and BBa_k in different substrates and temperatures. The PyeaR promoter showed a better activity over the HybB promoter.

17 FUTURE DEVELOPMENTS CspA promoter (BBa_K328001) OmpA + AoX enzyme
Nitrates biosensor (BBa_K381001)

18 PROJECT OBJECTIVES & OVERVIEW
PROJECT DESCRIPTION, EXPERIMENTS & RESULTS HUMAN PRACTICE PROJECT

19 PROBLEMS How to introduce Synthetic Biology into our society. Be able to find all the availability of institutions and the clear regulatory frame to develop our project. OBJECTIVES The development of an outreach campaign about synthetic biology basic topics for every kind of public. The development of a interactive software introducing the basics of synthetic biology and the iGEM competition. The development of a guidance software that assists the user in the execution of projects related with synthetic biology.

20 OUTREACH Synthetic Biology Tour with brochures, visuals, speeches and surveys.

21 SURVEY’S RESULTS

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23 Our team is also part of a new project called “Community Bricks”, a post iGEM group, that will be released this year. We are also participating in the set up of the Alumni iGEM.

24 This software is available on Visual Basic 2010.
SB UTP 1.0 SB UTP 1.0 is an interactive and educational program that introduces Synthetic Biology and iGEM to the users. This software is available on Visual Basic 2010.

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26 SB UTP PROJECT 1.0 SB UTP PROJECT 1.0 is a guidance program that helps groups of people with the process of implementing a Synthetic Biology project in their country.

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30 This software is available.
At the end of every question of the program, you will find a website or a paper as reference about the topic. Our future objective for this software is that it will end as a data base, so that teams of any part of the world could use it and also add more information. We exhort all of the future teams to complement this software. This software is available.

31 ASSOCIATE COLLABORATER
SPONSORS ASSOCIATE COLLABORATER


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