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UNDERGRADUATE STUDENTS: 1.Emmanuel Garuz 2.Fotis Stringos 3.Alicia Racine 4.Miguel Cardenas 5.Salomon Mitre 6.Valery Diaz 7.Naicolette A. Muñoz 8.Jose.

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Presentation on theme: "UNDERGRADUATE STUDENTS: 1.Emmanuel Garuz 2.Fotis Stringos 3.Alicia Racine 4.Miguel Cardenas 5.Salomon Mitre 6.Valery Diaz 7.Naicolette A. Muñoz 8.Jose."— Presentation transcript:

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3 UNDERGRADUATE STUDENTS: 1.Emmanuel Garuz 2.Fotis Stringos 3.Alicia Racine 4.Miguel Cardenas 5.Salomon Mitre 6.Valery Diaz 7.Naicolette A. Muñoz 8.Jose Ng 9.Arturo Nieves 10.Dukakis Tejada 11.Kesia Barrows 12.Michelle Camarena INSTRUCTOR & DIRECTOR Grimaldo Elías Ureña M. HUMAN PRACTICE ADVISOR Lucia L. Palma

4 Agricultural, Food & Civil-Environmental EngineeringAgricultural, Food & Civil-Environmental Engineering Mechanical and Material Sciences EngineeringMechanical and Material Sciences Engineering Electrical & Computational EngineeringElectrical & Computational Engineering Engineering tools obtained from biological resources

5 Design of powerful sensors Improvement of organisms ability to survive under temperature changes Focus on environmental and climatic issues. Applications

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7 Nitrates biosensor (BioBrick BBa_K381001) AOX periplasmatic heat generator (BioBrick BBa_K410000)

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9 Make Competent Cells using JM109 Test our Competent Cells Get Familiarized with Protocols New England Bio LabsNew England Bio Labs Open Wet WareOpen Wet Ware Reagents and buffer preparationReagents and buffer preparation TransformationTransformation Mini PrepMini Prep ElectrophoresisElectrophoresis DigestionDigestion LigationLigation

10 Test operation using 40mM KNO (if needed) under different: Substrates: LB Minimal essential medium Saline solution Temperatures: 8°C 23°C 37°C Improve the characterization of pre-existing BioBricks

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16 Determining the experimental conditions for optimal results for The Renbo. Best growth in Improving characterization of pre-existing BioBricks New results for BBa_K and BBa_k in different substrates and temperatures. The PyeaR promoter showed a better activity over the HybB promoter.

17 CspA promoter (BBa_K328001)CspA promoter (BBa_K328001) OmpA + AoX enzymeOmpA + AoX enzyme Nitrates biosensor (BBa_K381001)Nitrates biosensor (BBa_K381001)

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19 1. How to introduce Synthetic Biology into our society. 2. Be able to find all the availability of institutions and the clear regulatory frame to develop our project. 1.The development of an outreach campaign about synthetic biology basic topics for every kind of public. 2.The development of a interactive software introducing the basics of synthetic biology and the iGEM competition. 3.The development of a guidance software that assists the user in the execution of projects related with synthetic biology.

20 Synthetic Biology Tour with brochures, visuals, speeches and surveys.

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23 Our team is also part of a new project called Community Bricks, a post iGEM group, that will be released this year. We are also participating in the set up of the Alumni iGEM.

24 SB UTP 1.0 is an interactive and educational program that introduces Synthetic Biology and iGEM to the users. This software is available on Visual Basic 2010.

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26 SB UTP PROJECT 1.0 is a guidance program that helps groups of people with the process of implementing a Synthetic Biology project in their country.

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30 Our future objective for this software is that it will end as a data base, so that teams of any part of the world could use it and also add more information. At the end of every question of the program, you will find a website or a paper as reference about the topic. We exhort all of the future teams to complement this software.

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