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ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

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Presentation on theme: "ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)"— Presentation transcript:

1

2 ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

3 Reading Graphs Which graph shows the amount of substrate going to zero faster? Time (sec) Substrate Time (sec) Substrate

4 So… Nope, the amount of substrate gets to zero in less time in the OTHER graph. Time (sec) Substrate Time (sec) Substrate

5 So… YES!! The amount of substrate gets to zero in less time in this graph. Time (sec) Substrate Time (sec) Substrate

6 So… Which enzyme works FASTER? Time (sec) Substrate Time (sec) Substrate

7 So… Nope, not this one. The faster the enzyme works, the faster the substrate will decrease. Time (sec) Substrate Time (sec) Substrate

8 So… YES!! This enzyme works faster because the substrate decreases faster!! Time (sec) Substrate Time (sec) Substrate

9 What do you think can affect the speed of an enzyme? TEMPERATURE AMOUNT OF LIGHT pH

10 What do you think can affect the speed of an enzyme? RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme. ANYTHING ELSE? RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme. ANYTHING ELSE? TEMPERATURE AMOUNT OF LIGHT pH

11 What do you think can affect the speed of an enzyme? RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme. TEMPERATURE AMOUNT OF LIGHT pH

12 What do you think can affect the speed of an enzyme? RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. ANYTHING ELSE? RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. ANYTHING ELSE? TEMPERATURE AMOUNT OF LIGHT pH

13 What do you think can affect the speed of an enzyme? RIGHT!! Changing the pH of the reaction can ALSO affect the speed of an enzyme. TEMPERATURE AMOUNT OF LIGHT pH

14 What do you think can affect the speed of an enzyme? Sorry, most enzymes are NOT affected by the amount of light… Pick again Sorry, most enzymes are NOT affected by the amount of light… Pick again TEMPERATURE AMOUNT OF LIGHT pH

15 Factors affecting enzyme speed We can make a graph of enzyme speed at different temperatures.

16 Graph of ENZYME SPEED Speed of enzymeTemperature (°C) What should go on the X axis?

17 Graph of ENZYME SPEED Speed of enzymeTemperature (°C) Nope, the speed of the enzyme is what we are MEASURING,it should go on the Y axis.

18 Graph of ENZYME SPEED RIGHT! Temperature should go on the X axis. Temperature (°C) What should go on the Y axis? (HINT: This is what we are MEASURING) Amount of substrate Speed of enzyme

19 Graph of ENZYME SPEED Temperature (°C) What should go on the Y axis? (HINT: This is what we are MEASURING) We’re not measuring the amount of substrate this time. Amount of substrate Speed of enzyme

20 Graph of ENZYME SPEED Temperature (°C) RIGHT!! The speed of enzyme should go on the Y axis. Speed of enzyme REMEMBER!! The enzyme is working FASTER As you go UP the Y axis. SLOW FAST

21 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase is found in saliva. Click on the red button that is approximately at body temperature

22 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase is found in saliva. 5°C is close to freezing. TOO COLD!!

23 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase is found in saliva. 22°C is close to room temperature. TOO COLD!!

24 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase is found in saliva. 55°C is 131°F. TOO HOT!!

25 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase is found in saliva. 37°C is body temperature. RIGHT!! Amylase will work best at about 37°C because it is normally found in your mouth

26 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase works slowly At very low temperatures Because it is not close To body temperature

27 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase will work Faster as it gets Closer to body Temperature.

28 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Amylase will work fastest at body Temperature. The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE

29 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST As the temperature gets too hot, Amylase doesn’t work as well.

30 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Click on the OPTIMUM TEMPERATURE

31 Let’s GRAPH!! Temperature (°C) Speed of enzyme SLOW FAST Click on the OPTIMUM TEMPERATURE RIGHT!!

32 What is the optimum temperature for this enzyme? Temperature (°C) Speed of enzyme SLOW FAST 10°C 20°C 30°C

33 What is the optimum temperature for this enzyme? Temperature (  C) Speed of enzyme SLOW FAST 10°C 20°C 30°C REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at.

34 What is the optimum temperature for this enzyme? Temperature (°C) Speed of enzyme SLOW FAST 10°C 20°C 30°C REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at.

35 Right!!

36 What is the optimum pH for this enzyme? pH Speed of enzyme SLOW FAST

37 What is the optimum pH for this enzyme? pH Speed of enzyme SLOW FAST Look closely…

38 What is the optimum pH for this enzyme? pH Speed of enzyme SLOW FAST Look closely…

39 Right!!

40 What is the optimum temperature for this enzyme? Temperature (°C) Speed of enzyme SLOW FAST 20°C 63°C 48°C

41 What is the optimum temperature for this enzyme? Temperature (°C) Speed of enzyme SLOW FAST 20°C 63°C 48°C

42 What is the optimum temperature for this enzyme? Temperature (°C) Speed of enzyme SLOW FAST 20°C 63°C 48°C

43 Right!!

44 What temperature does the enzyme work best at? Time Substrate Temperature = 25°C Time Substrate Temperature = 35°C Time Substrate Temperature = 30°C At 25°CAt 30°CAt 35°C

45 REMEMBER: the faster the substrate decreases, the faster the enzyme is working Time Substrate Temperature = 25°C Time Substrate Temperature = 35°C Time Substrate Temperature = 30°C At 25°CAt 30°CAt 35°C

46 REMEMBER: the faster the substrate decreases, the faster the enzyme is working Time Substrate Temperature = 25°C Time Substrate Temperature = 35°C Time Substrate Temperature = 30°C At 25°CAt 30°CAt 35°C

47 TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ

48 QUIZ - QUESTION 1 Where in your body is the amylase enzyme found? SALIVASTOMACHLIVER

49 QUIZ - QUESTION 1 Where in your body is the amylase enzyme found? SALIVASTOMACHLIVER

50 QUIZ - QUESTION 1 Where in your body is the amylase enzyme found? SALIVASTOMACHLIVER

51 Right!!

52 QUIZ - QUESTION 2 Click on the CATALYST in this reaction: STARCH SUGAR AMYLASE

53 QUIZ - QUESTION 2 REMEMBER: The catalyst performs the reaction, but isn’t used up STARCH SUGAR AMYLASE

54 QUIZ - QUESTION 2 REMEMBER: The catalyst performs the reaction, but isn’t used up STARCH SUGAR AMYLASE

55 Right!!

56 QUIZ - QUESTION 3 Click on the substrate that is most likely to react with the enzyme below: PROTEINLIPIDSTARCH

57 QUIZ - QUESTION 3 REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. PROTEINLIPIDSTARCH

58 QUIZ - QUESTION 3 REMEMBER: The subtrate that fits into a protein will be the one that reacts with it. PROTEINLIPIDSTARCH

59 Right!! PROTEIN

60 QUIZ - QUESTION 4 What is the most likely name of the enzyme in question 3 that reacts with protein? PROTEINOSE PROTEINASEPROTEINZYME PROTEINATION

61 QUIZ - QUESTION 4 NOPE: sugars end in -OSE, not enzymes (sucrose, lactose, glucose) PROTEINOSE PROTEINASEPROTEINZYME PROTEINATION

62 QUIZ - QUESTION 4 NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINASEPROTEINZYME PROTEINATION

63 QUIZ - QUESTION 4 NOPE: think about “lactase” and “amylase” PROTEINOSE PROTEINASEPROTEINZYME PROTEINATION

64 Right!!

65 QUIZ - QUESTION 5 Click on the part of the graph that shows the OPTIMAL temperature for this enzyme Temperature (°C) Enzyme Activity

66 QUIZ - QUESTION 5 REMEMBER: the OPTIMAL temperature for an enzyme is the temperature where the enzyme works BEST (highest activity) Temperature (°C) Enzyme Activity

67 Right!!

68 QUIZ - QUESTION 6 Click on the graph that shows the optimum pH for this enzyme. Time Substrate Time Substrate Time Substrate pH = 5.0 pH = 3.0pH = 7.0

69 QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works faster Time Substrate Time Substrate Time Substrate pH = 5.0 pH = 3.0pH = 7.0

70 QUIZ - QUESTION 6 REMEMBER: Substrate decreases in LESS time if the enzyme works faster Time Substrate Time Substrate Time Substrate pH = 5.0 pH = 3.0pH = 7.0

71 Right!!

72

73 You have successfully completed the enzyme powerpoint.


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