1. A. B. Online resource 7 Soria G. et al. Submitted to Neoplasia
Title of manuscript:
The secretion of the chemokine CCL5 by breast tumor cells is regulated by the 40s loop of the chemokine and by intracellular glycosaminoglycans A. B.
p=0.04
p=0.02
Relative detection of GFP-CCL5 release (%)
CCL5 Extracellular Expression (pg/107 cells)
GFP-CCL5
(WT)
GFP-CCL5
(TRKN--)
GFP-CCL5
(WT)
GFP-CCL5
(TRKN--)
Further evidence to prominent reduction in secretion of GFP-CCL5(TRKN--) compared to GFP-CCL5(WT) by breast tumor cells Human MCF-7 breast tumor cells were transiently transfected by vectors expressing GFP-CCL5(WT) or GFP-CCL5(TRKN--), followed by determination of chemokine expression in cell supernatants. (A) ELISA assays with mAb to GFP and polyclonal detecting antibodies to CCL5 (“Procedure 3” in “Materials and methods”). (B) ELISA assays with polyclonal coating antibodies to CCL5 and polyclonal detecting antibodies to CCL5 (“Procedure 4” in “Materials and methods”).