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Validation of a point-of-care desk top device to quantitate fecal calprotectin and distinguish inflammatory bowel disease from irritable bowel syndrome 

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Presentation on theme: "Validation of a point-of-care desk top device to quantitate fecal calprotectin and distinguish inflammatory bowel disease from irritable bowel syndrome "— Presentation transcript:

1 Validation of a point-of-care desk top device to quantitate fecal calprotectin and distinguish inflammatory bowel disease from irritable bowel syndrome  Michael J. Sydora, Beate C. Sydora, Richard N. Fedorak  Journal of Crohn's and Colitis  Volume 6, Issue 2, Pages (March 2012) DOI: /j.crohns Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

2 Figure 1 Correlation of calprotectin concentrations with age and clinical activity as determined by HBI score (CD) and Mayo score (UC). Each dot represents the concentration of fecal calprotectin as determined with the Bühlmann Quantum Blue Reader® in individual UC, CD, and IBS patients and control subjects without intestinal diseases. Numbers represent clinical activity scores and open circles represent patients with recent surgery. The dotted line denotes the mean value in each group. Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns ) Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

3 Figure 2 Comparison of calprotectin levels measured with the point-of-care desk-top Bühlmann Quantum Blue Reader® (A) with those measured by standard ELISA techniques (B). Data represent the mean±SEM from samples of controls (n=19), IBS (n=7), CD (n=7), and UC (n=9) patients. **p<0.05 compared with both IBS and controls,*p<0.05 compared with controls. Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns ) Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

4 Figure 3 Direct comparison of results from standard ELISA and point-of-care desk-top Bühlmann Quantum Blue Reader® (QBR). Each line connects the level of calprotectin measured with the Quantum Blue Reader® to the level measured with standard ELISA techniques. CD and UC Patients that had undergone recent surgery are marked with S. The cutoff line for results derived from measurements by standard ELISA and Quantum Blue Reader® are marked with dotted lines accordingly. Journal of Crohn's and Colitis 2012 6, DOI: ( /j.crohns ) Copyright © 2011 European Crohn's and Colitis Organisation Terms and Conditions

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