1. Bone metabolism in obese rats programmed by early weaning
Lígia de Albuquerque Maia, Patrícia Cristina Lisboa, Elaine de Oliveira, Natália da Silva Lima, Inaya Correa Barbosa Lima, Ricardo Tadeu Lopes, Leandro Dias Gonçalves Ruffoni, Keico Okino Nonaka, Egberto Gaspar de Moura 
Metabolism - Clinical and Experimental 
Volume 63, Issue 3, Pages (March 2014)
DOI: /j.metabol
Copyright © 2014 Elsevier Inc. Terms and Conditions

2. Fig. 1
(a) Computed tomography image of femur, the region of interest is shown by R1 (femoral head); (b) Computed tomography image of lumbar vertebra, the region of interest is shown by R2 (vertebral body); (c) 3D representative microcomputed tomography image of the cylindrical region of interest (1.86mm of diameter) at the center of the femoral head showing the region of trabecular bone studied.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

3. Fig. 2
Body weight after weaning until pups of 180days of control (•), mechanically early-weaned (■) and pharmacologically early weaned (▲).* MEW mean values were significantly different from those of C (P<0.05); †PEW mean values were significantly different from those of C (P<0.05), n=8.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

4. Fig. 3
(a) Accumulated ingestion; (b) Feed efficiency at 180 days; (c) Visceral fat mass; (d) Serum leptin of control (C), mechanically early-weaned (MEW) and pharmacologically early weaned (PEW) offspring. Values are means, with their standard errors represented by vertical bars, n 8. *Mean values were significantly different from those of C (P<0.05). †Mean values were significantly different from those of MEW (P<0.05).
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

5. Fig. 4
(a) Total BMD; (b) Total BMC; (c) Spine BMD; (d) Bone Area of control (•), mechanically early-weaned (■) and pharmacologically early weaned (▲) offspring at different ages (90, 120, 150 and 180days). *Mean values were significantly different from those of control (P<0.05); †Mean values were significantly different from those of MEW (P<0.05). n=8 animals/group.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

6. Fig. 5
(a) Femur BMD; (b) Fourth lumbar vertebra BMD; (c) Femoral head radiodensity; (d) Fourth lumbar vertebra vertebral body radiodensity at 180 days of control (C), mechanically early-weaned (MEW) and pharmacologically early weaned (PEW) offspring; (a) and (b) were evaluated by DXA and (c) and (d) were evaluated by CT. Values are means, with their standard errors represented by vertical bars. *Mean values were significantly different from those of C (P<0.05). †Mean values were significantly different from those of MEW (P<0.05). n=8 animals/group.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

7. Fig. 6
(a) Bone volume fraction (BV/TV); (b) Trabecular Pattern Factor (Tb.Pf); (c) Trabecular Thickness (Tb.Th); (d) Trabecular Number (Tb.N); (e) Trabecular Separation (Tb.Sp) at 180days of control (C), mechanically early-weaned (MEW) and pharmacologically early weaned (PEW) offspring, evaluated by micro-CT. Values are means, with their standard errors represented by vertical bars. *Mean values were significantly different from those of C (P<0.05); †Mean values were significantly different from those of MEW (P<0.05). n=8 animals/group.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

8. Fig. 7
(a) Maximal Deformation; (b) Stiffness; (c) Break Load; (d) Break Deformation at 180days of control (C), mechanically early-weaned (MEW) and pharmacologically early weaned (PEW) offspring, evaluated by three-point bending test. Values are means, with their standard errors represented by vertical bars. *Mean values were significantly different from those of C (P<0.05). n=8 animals/group.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions

9. Fig. 8
(a) CTX-I; (b) 25-Hydroxyvitamin D3 of control (C); mechanically early-weaned (MEW) and pharmacologically early-weaned (PEW) offspring. Values are means, with their standard errors represented by vertical bars. *Mean values were significantly different from those of C (P<0.05). n=8 animals/group.
Metabolism - Clinical and Experimental  , DOI: ( /j.metabol )
Copyright © 2014 Elsevier Inc. Terms and Conditions