1. Fig. 6. Expression of CD69 and CD25 under vitamin C treatment at various times before and after activation. Human T cells (5×105 cells/well) were cultured for 2 hours in a 24-well plate with 1 mM vitamin C before phorbol-12-myristate-13-acetate/ionomycin activation and 3, 6, and 12 hours after activation Twenty-four hours after activation, cells were harvested, stained with appropriate antibodies, and were subjected to flow cytometric analysis. The experiment was repeated three times and a representative one is shown. Numbers are in %. SSC, side-scattered light; FSC, forward-scattered light.
Fig. 6. Expression of CD69 and CD25 under vitamin C treatment at various times before and after activation. Human T cells (5×105 cells/well) were cultured for 2 hours in a 24-well plate with 1 mM vitamin C before phorbol-12-myristate-13-acetate/ionomycin activation and 3, 6, and 12 hours after activation Twenty-four hours after activation, cells were harvested,. . .
Anat Cell Biol Jun;49(2):