1. Role of caspases on cell death, inflammation, and cell cycle in glycerol-induced acute renal failure 
E. Homsi, P. Janino, J.B.L. de Faria 
Kidney International 
Volume 69, Issue 8, Pages (April 2006)
DOI: /sj.ki
Copyright © 2006 International Society of Nephrology Terms and Conditions

2. Figure 1
Evaluation of acute tubular necrosis (ATN) in Gly-ARF 5 ml/kg at 24 h. The pan-caspase inhibitor (Z-VAD-FMK) treated rats had less ATN than untreated rats. *P<0.05 vs Gly-ARF.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

3. Figure 2
Evaluation of tubular cell apoptosis (TUNEL positive cells) in Gly-ARF 5 ml/kg. (a) Number of TUNEL positive cells significantly increased in the renal medulla 6 h after Gly-ARF induction. N: 5 rats/group. (b) Separate experiment showing that Z-VAD-FMK significantly reduced the number of TUNEL positive cells 6 h after Gly-ARF 5 ml/kg. N: 5 rats/group. *P<0.05 vs other groups.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

4. Figure 3
Evaluation of immunohistochemistry for IL-1β and IL-18 in the renal parenchyma. IL-1β immunostaining observed at low power field (× 40) in (a) control rat and (b) Gly-ARF. IL-1β expression predominates in the renal cortex and is enhanced in Gly-ARF. At higher magnification (× 400), IL-1β expression in (c) control rat and (d) Gly-ARF showing tubule cells positivity. The expression of IL-18 in (e) control rat and (f) Gly-ARF were similar and observed in the cortex and outer medulla tubule cells.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

5. Figure 4
Western blot from cortical kidney homogenates showing the expression of IL-1β and IL-18. (a) The IL-1β inactive 35 kDa precursor was detected in controls (C), Gly-ARF (Gly), and Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK), whereas the 17 kDa active IL-1β form was detected in Gly-ARF and absent in Gly-ARF+Z-VAD-FMK. (b) The IL-18 inactive 24 kDa precursor was detected in controls (C), Gly-ARF (Gly), and Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK), whereas the 18 kDa active IL-18 form was detected in control and Gly-ARF and absent in Gly-ARF+Z-VAD-FMK.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

6. Figure 5
Number of BrdU positive cells in renal parenchyma 48 h after Gly-ARF. There was a significant increase in BrdU positive cell (mitosis) in Gly-ARF. Z-VAD-FMK treated rats showed positivity similar to untreated Gly-ARF. N: 6 rats/group, *P<0.001 vs control.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

7. Figure 6
Western blot from cortical kidney homogenates showing expression of p27Kip detected in controls (c) and absent in Gly-ARF (Gly) or Gly-ARF treated with Z-VAD-FMK (Gly+Z-VAD-FMK).
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions