1. Variants near the HLA complex group 22 gene (HCG22) confer increased susceptibility to late-onset asthma in Japanese populations 
Yohei Yatagai, MD, PhD, Tomomitsu Hirota, DDS, PhD, Tohru Sakamoto, MD, PhD, Hideyasu Yamada, MD, Hironori Masuko, MD, PhD, Yoshiko Kaneko, MD, PhD, Hiroaki Iijima, MD, PhD, Takashi Naito, MD, PhD, Emiko Noguchi, MD, PhD, Mayumi Tamari, MD, PhD, Michiaki Kubo, MD, PhD, Atsushi Takahashi, PhD, Satoshi Konno, MD, PhD, Hironi Makita, MD, PhD, Masaharu Nishimura, MD, PhD, Minako Hijikata, MD, PhD, Naoto Keicho, MD, PhD, Sakae Homma, MD, PhD, Yoshio Taguchi, MD, Arata Azuma, MD, PhD, Shoji Kudoh, MD, PhD, Nobuyuki Hizawa, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 138, Issue 1, Pages e13 (July 2016)
DOI: /j.jaci
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2. Fig 1
ORs for association between rs and age of onset–specific subsets of asthma. By using 548 asthma cases and 1668 control subjects who had accurate information on age of onset of asthma in discovery sample 1 and the replication sample, the OR (95% CI) for association was plotted between rs and age of onset–specific subsets of asthma. The numbers of asthmatic patients who had the disease at the ages of 10 years or later, 20 years or later, 30 years or later, 40 years or later, 50 years or later, and 60 years or later were 457, 437, 379, 301, 238, and 153, respectively. ORs significantly greater than 1 are highlighted. *P < .005, **P < .001, and ***P < .0005.
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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3. Fig E1 Flow chart of the study.
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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4. Fig E2
Manhattan plots of the distribution of −log10 (P values) for association of 473,981 SNPs with susceptibility to late-onset asthma in the genome-wide meta-analysis. The x-axis represents the chromosomal position.
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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5. Fig E3
Age of asthma onset according to the genotype at rs Using 548 patients with asthma in discovery sample 1 and the replication sample who had accurate information on age of asthma onset, we compared age of onset according to the genotype at rs using the Kruskal-Wallis test, which showed no significant differences among the 3 genotypes (P = .06).
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6. Fig E4
Location of rs and rs on chromosome 6p21.3. The positions of rs and rs on chromosome 6p21.3 based on GRCh38.p2 are 31,046,339 and 31,041,041 bp, respectively. The distances between these SNPs and the transcription initiation site (31,053,540 bp) of HCG22 are 7,201 and 12,499 bp for rs and rs , respectively.
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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7. Fig E5
Regional association plot and schematic diagram of linkage disequilibrium blocks in the mucin-like gene cluster region on chromosome 6p21.3. P values of the genotyped SNPs (red circles) and imputed SNPs (purple circles) are plotted against their physical position on chromosome 6 (NCBI36/hg18). Black triangles represent linkage disequilibrium blocks. Red, D′ ≥ 0.80; pink, 0.5 ≤ D′ < 0.8; blue, 0.2 ≤ D′ < 0.5; white, D′ < 0.2.
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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8. Fig E6
Fine plots of the distribution of −log10 (P values) for association of SNPs with HCG22 mRNA expression. rs and rs were closely located between HCG22 and MUC22 and showed strong associations with HCG22 mRNA expression (P = 8.54 × 10−87 and 3.23 × 10−84, respectively).
Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci )
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