We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published bySamuel Lynch
Modified over 2 years ago
Suppl. Fig. 1 AB HPDE PANC1 Suit028 Suit007CapanI PaTu 8988t MonoMac 6 TGF Actin
A Suppl. Fig. 2 empty vector CUX1 β-Actin E D CUX1 β -Actin untreatedCtrl siRNACUX1 siRNA #1 CUX1 siRNA #2 CUX1 siRNA #3 BC CXCL10CCL5
NFkB p LVCUX1 CUX1 STAT1 β-Actin STAT3 STAT6 NFkB p50 Phospho Stat3 (Ser727) phosphoNFkBp65 (Ser536) A Suppl. Fig. 3 TNF (incubation time in hours)
β -Actin STAT1 STAT3 STAT6 LV- LV+CUX- CUX+ LV-LV+ CUX- CUX+ Nucleus Cytoplasma CUX1 MEK1 ORC2 B Suppl. Fig. 3
Suppl. Fig. 4 human mouse -200 ACCTCACTCGTTAAAAATTAAAAGGAGCACAAGAGGGG-AGAGGGAAATTCC--AAGTTCATGGGTCACAATAAACACAAGCAATGCCCTCG-GTTT---ACAGGGGACTTCCCTCGGGT -212 ACCTAATTCACTATTACCAAAAA AAGAGGAGCAGAGGGAAATTCCGTAACTTGGAGGCT-ACAATAAATA------ATACCTTCGAGTCTGCAACATGGGACTTCCCCAGGA---- human mouse T-GC--GGAGCCTT-GCTGAGTCATCTCCAAAGTC---AGCCAATCAGGACTCAGGGAGGGAAACT--CTTTGCAGATAAATA---CTC -10 ACAGCCAGCAGGTTTTGCTAAGTCAACTGTAATGCCCTTATCCAATCAGAATT-AGGGAGGGAAAATGGCTTTGCAGATAAATATGGCAC -18 CXCL10 CXCL10-1 for CXCL10-1 rev CXCL10-2 for CXCL10-2 rev Oligo-1 Oligo-2 Green: putative CUX1 binding site Red: putative NF-kB binding sites Solid lines: qPCR primer for ChIP Dashed lines: Oligonucleotides for DNA-pulldown
Suppl. Fig. 5 A * BC merge DAPI Granzyme B CD8
SKOV-3, 48h CaOV-3, 48h BG-1, 48h Concentration (µM) Viable cells (%) SW 43 SW IV-52s SW III-123 SW 43 + SW IV-52s SW 43 SW IV-52s SW III-123 SW 43 + SW.
ScrMMP-1 Supplementary Fig. 1, Ganguly et al ScrMMP-3 ScrMMP-3MT1-MMP 100 m Relative Invasion Scr MMP m abc Scr Scr.
Supplemental Figure 1 a % pulldown from input LNCaP/scrambled-siRNA Figure 1 A, ChIP analysis of AR and PHB binding to the PSA promoter in the LNCaP/scrambled-siRNA.
Supplementary Figure 1. Caspase 8 expression and activation and Fas expression in NSCLC and normal lung cell lines. A Western blot analysis of DR4 and.
Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **
Supplementary Figure S1: The hair cycle The hair follicle undergoes a continuous cyclical process after morphogenesis involving regression and regeneration.
PI Cell number proliferating A PI Cell number PI Cell number PI Cell number senescent A549 proliferating MCF-7senescent.
Nano Curcumin for Breast Cancer Prevention Banu Arun, M.D. Professor, Breast Medical Oncology Co-Director Clinical Cancer Genetics.
Cellecta, Inc. Targets & Tools March 20/21, 2012 Cellecta, Inc. Targets & Tools March 20/21, 2012 Paul Diehl, Ph.D. Director of Business Development RNAi.
1 Ottobre 22, 2008 © 2008 Applied BiosystemsInternational School of Functional Genomics Click to edit Master title style Click to edit Master subtitle.
Useful hints and tips for cloning, PCR and site-directed mutagenesis Kirsten Jensen Division of Molecular Biosciences.
NEGpre-221 pre-222pre-221/222 NEG DMSO 100 nM Fulvestrant for 72h Figure S1. Cell morphology of MCF7 cells after treatment with fulvestrant, with or without.
Overview of Our Integrated Proteomics and Bioinformatics Services Platform The Systems Proteomics Company 2011 March 25.
Supplementary Figure 1 b) Human miRNA-199a - Axlc) Human miRNA-199b - Axl a) Human miRNA-34a - Axl Predicted secondary structures (Pic Tar predicitions)
Figure S1 Approximate positions of primers listed in Supplementary Table S1 for F3H (a), Tdic101 (b) and dTdic102 (c). Black arrow heads show primers and.
Chr6 p11.2 B A Gene density Log2 (H2AX/input) Log2 (mock/input) C 53 MB 55 MB 57 MB 200 MB 202 MB 204 MB 206MB Log2(H2AX/input)
C E N T R F O R I N T E G R A T I V E B I O I N F O R M A T I C S V U E Bioinformatics Master Course: DNA/Protein Structure-Function Analysis and Prediction.
Regolazione dellespressione genica. CAP Cytoplasm Nucleus DNA RNA Processing Export Degradation Rat1p Exos. Nuclear pore AAAAAA Rrp6p ?
Figure S1. In absence of Rvb1, treatment with various DNA damaging agents results in increased phosphoH2AX. HeLa cells were transfected with indicated.
September 8, 2011 "The way to be nothing is to do nothing." -- Nathaniel Howe Test prep, p. 18 #
Bell Ringers Week 12. Which choice represents the solution of the equation -3(x + 2) = 5x? a) c) b) d) No Solution.
1 Cytokine Expression in the Central Nervous System and Periphery during Infection with Rabies Virus Nicholas Johnson Rabies and Wildlife Zoonoses Group.
1 REAL TIME PCR USING SYBR GREEN. 2 THE PROBLEM NEED TO QUANTITATE DIFFERENCES IN mRNA EXPRESSION SMALL AMOUNTS OF mRNA –LASER CAPTURE –SMALL AMOUNTS.
1 Fig. S1 HeLa cells ROS dye added after trypsinization ROS dye added before trypsinization Percentage of DCF fluorescence positive cells (H 2 O 2 production)
Supp Figure S1, Yang et al.Supp Figure S2, Yang et al. Supp Figure S1. Calorimetric analysis of La(1-194) and LARP4( ) interactions with single stranded.
Paul M. Yen, M.D. Laboratory of Hormonal Regulation Cardiovascular and Metabolic Diseases Program Duke-NUS Graduate Medical School
A b Supp. Figure 1: Anoikis-resistance of MDA-MB-231 is dependent on PI3Kγ (a) Cells were prevented from attaching for 48 hours in the presence of DMSO,
Blotting Techniques: Limitations needs g amounts of DNA DNA needs to be relatively pure assay time: several days to > week specific DNA fragment(s) are.
Pp65-specific all B: CD8 T-cells CD8/pp65 T cells per ml blood A: CD8 T-cells Indicated memory subset as a proportion of the reference T-cell subset FIG.
1 Chapter 27 Transcription Factors in the Central Nervous System Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All.
© 2016 SlidePlayer.com Inc. All rights reserved.