1. Figure 1 C. LDH release D. Cell viability E. Cell proliferation
(LDH assay, O.D.)
D. Cell viability
***
(MTT test, O.D.)
E. Cell proliferation
(BrdU incorporation, O.D.)
B. Cell death area
(mm2)
A. Wound closure
Hyperoxia
(mm2 per 24 h)
0.1
0.2
0.3
0.4
0.5
0.25
0.75 1
1.25
0,05
0,1
0,15
Control in
room air
Figure 1

2. Figure 2 C. LDH release B. Cell death area A. Wound closure
D. Cell viability
E. Cell proliferation * 20 40 60 80
100
120
140
160
180 + 25 50 75
125
***
Control in room air
Hyperoxia
+ KGF 10ng/ml
+ KGF 100ng/ml
150
200
250
300
350
400
500
600
700
800
900
1000
1100
+++
Figure 2

3. Survival rate
Time (days)
0.2
0.4
0.6
0.8 1 2 4 6 8 10
Figure 3

4. Figure 4 Cells in BAL (x 10-4 / ml) ** 22.5 20 17.5 15 12.5 10 7.5 5
Control
KGF
Control
KGF
Room air
Hyperoxia
Figure 4

5. Figure 5 DNA content (µg / mg ww) *** 8 7 6 5 4 3 2 1 Room air1 2 3 4 5 6 7 8
DNA content (µg / mg ww)
Hyperoxia
***
Figure 5

6. D C B A
100 µm
Figure 6

7. Figure 7 Caspase-3 protein *** § day 5 day 10
room air
hyperoxia
day 5
day 10
Caspase-3 protein 20 40 60 80
100
120
% of day 5 room-air control
35kDa
19kDa
17kDa
Ponceau S
Figure 7