1. Figure 5. Effect of sugars on cAspAT mRNA in 3T3-F442A adipocytes
Figure 5. Effect of sugars on cAspAT mRNA in 3T3-F442A adipocytes. 3T3-F442A adipocytes were maintained for 24 h in culture medium in which glucose was either omitted (CTL) or changed to 25 mm of the indicated sugars. RNA was analyzed by Northern hybridization with a labeled cAspAT cDNA probe. Data obtained by scanning densitometry, was normalized for RNA loading using theβ -Actin mRNA signal. Data are expressed as the percent of cAspAT signal from 24-h glucose-deprived cells. Each value represents the mean ± sem of data obtained from at least three independent experiments with duplicate dishes. d-gluc, d-glucose; l-gluc, l-glucose; 3-OMG, 3-orthomethyl glucose; 2-DOG, 2-deoxyglucose.
Identification of an Adipocyte-Specific Negative Glucose Response Region in the Cytosolic Aspartate Aminotransferase Gene**This work was supported by Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, and the Université Paris-Val de Marne.
Endocrinology. 1998;139(12): doi: /endo
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