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Feeding with olive oil attenuates inflammation in dextran sulfate sodium-induced colitis in rat
Toru Takashima, Yasuhisa Sakata, Ryuichi Iwakiri, Ryosuke Shiraishi, Yasutomo Oda, Norie Inoue, Atsushi Nakayama, Shuji Toda, Kazuma Fujimoto Journal of Nutritional Biochemistry Volume 25, Issue 2, Pages (February 2014) DOI: /j.jnutbio Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 1 Experiment design. DSS was administered to rats via drinking water (weight percentage: 4%) for a 1-week period followed by a 1-week recovery period. This cycle was repeated three times. Rats were divided into three groups at random as shown: control group, standard diet and no DSS; DSS group, standard diet+DSS; and DSS+EVOO group, 5% olive oil diet+DSS. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 2 Changes in body weight of control group, DSS group and DSS+EVOO group. Rats were weighted every week from the start of treatment. Data are expressed as the means±S.E.M. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 3 DAI of control group, DSS group and DSS+EVOO group. The DAI consisted of three scales: presence of rectal bleeding, weight loss and stool consistency. Rats were weighted every week from the start of treatment. Data are expressed as the means±S.E.M. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 4 Macroscopic view and lengths of colon on day of sacrifice. (A) Control group; (B) DSS group; (C) DSS+EVOO group. Data are expressed as the means±S.E.M. ⁎P<.01 vs. control. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 5 Representative images showing colon segments on day of sacrifice. (A) Control group, (B) DSS group, (C) DSS+EVOO group. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 6 Histological scores on day of sacrifice. Histological scores of proximal, middle and distal colon segments of control group, DSS group and DSS+EVOO group. Data are expressed as the means±S.E.M. ⁎P<.01 vs. control. ⁎⁎P<.05 vs. DSS rats. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 7 Expressions of STAT3 and pSTAT3. Effects of 5% EVOO diet on STAT3 and pSTAT3 proteins using anti-STAT3 and anti-pSTAT3 antibodies. Control group; DSS group; DSS+EVOO group. The bands were quantified. β-Actin was used as the loading control. Data are expressed as the means±S.E.M. ⁎P<.01 vs. control. ⁎⁎P<.05 and ⁎⁎⁎P<.01 vs. DSS rats. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 8 Expressions of inflammatory proteins. Effects of 5% EVOO diet on expressions of inflammatory proteins using anti-COX-2 and anti-iNOS antibodies. (A) Images of each protein. Lanes A–C, control group; DSS group; DSS+EVOO group. (B) The bands were quantified. β-Actin was used as the loading control. Data are expressed as the means±S.E.M. ⁎P<.01 vs. control. ⁎⁎P<.01 vs. DSS rats. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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Fig. 9 Cell proliferation and apoptosis. Effects of 5% EVOO diet on cell proliferation and apoptosis using anti-PCNA and anti-cleaved caspase-3 antibodies. (A) Images of each protein. Lanes A–C, control group; DSS group; DSS+EVOO group. (B) The bands were quantified. β-Actin was used as the loading control. Data are expressed as the means±S.E.M. ⁎P<.05 and ⁎⁎P<.01 vs. DSS rats. Journal of Nutritional Biochemistry , DOI: ( /j.jnutbio ) Copyright © 2014 Elsevier Inc. Terms and Conditions
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