1. Novel remodeling of the mouse heart mitochondrial proteome in response to acute insulin stimulation 
B.A. Pedersen, P.G. Yazdi, J.F. Taylor, O.S. Khattab, Y.-H. Chen, Y. Chen, P.H. Wang 
Nutrition, Metabolism and Cardiovascular Diseases 
Volume 25, Issue 12, Pages (December 2015)
DOI: /j.numecd
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

2. Figure 1
Time course of phospho-Akt1 translocation to mitochondria upon in vivo insulin stimulation in the heart. Representative Western blots depicting phospho-Akt1 and Mn-SOD2 protein abundance in isolated heart mitochondria. Each time point is of a pool of mitochondria from 4 animals. Immunoblot with anti-Mn-SOD2 served as a loading control.
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

3. Figure 2
Two-dimensional differential electrophoresis of insulin stimulated cardiac mitochondria. Overlay of insulin stimulated vs. control. 1) actin, alpha cardiac muscle 1/ATP synthase subunit β, mitochondrial; 2) actin, alpha cardiac muscle 1; 3) succinyl-CoA ligase [ADP-forming] subunit β; 4) actin, alpha cardiac muscle 1; 5) tropomyosin α-1 chain; 6) myosin light chain 3; 7) myosin light chain 3; 8) myosin regulatory light chain 2, ventricular cardiac muscle isoform; 9) myosin regulatory light chain 2, ventricular cardiac muscle isoform. Additional information on spot identities and differential expression is in Table 1.
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

4. Figure 3
Acute insulin stimulation decreases the activity of ATP-specific succinyl-CoA synthetase in heart mitochondria. Shown are representative rates of conversion of succinyl-CoA to CoASH in mitochondrial pools from 4 animals as monitored by the reduction of DTNB. Observed rates were corrected for the nonenzymatic hydrolysis of succinyl-CoA and done in parallel with controls that demonstrated activity to be dependent on MgCl2 and ADP.
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

5. Supplemental Fig. 1
Blood glucose levels prior to and post oral feeding. Blood glucose levels increased following glucose feeding in comparison to water feeding (mean ± SD; 10 min water vs. glucose: 4.73 ± 1.12 vs. 9.01 ± 2.66; 30 min water vs. glucose: 4.28 ± 0.90 vs  ± 0.84; * denotes p < 0.05 by Student's t-test).
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

6. Supplemental Fig. 2
Two-dimensional differential electrophoresis (pH 3–7 NL) of heart mitochondria 10 min after oral glucose gavage. Overlay of glucose stimulated vs. control. 10) acyl carrier protein, mitochondrial. Additional information on spot identities and differential expression is in Supplemental Table 1.
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions

7. Supplemental Fig. 3
Two-dimensional differential electrophoresis (pH 3–7 NL) of heart mitochondria 30 min after oral glucose gavage. Overlay of glucose stimulated vs. control. 11) tropomyosin α-1 chain; 12) troponin T, cardiac muscle. Additional information on spot identities and differential expression is in Supplemental Table 1.
Nutrition, Metabolism and Cardiovascular Diseases  , DOI: ( /j.numecd )
Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University Terms and Conditions