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GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV A B CD 8 C CD 8 GFP GFP GFP-RV

Published byBenedict Russell Burns Modified over 6 years ago

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Presentation on theme: "GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV A B CD 8 C CD 8 GFP GFP GFP-RV"— Presentation transcript:

1 GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV A B CD 8 C CD 8 GFP GFP GFP-RV
MSCV MSCV GFP-RV 5‘ LTR IRES GFP 3‘ LTR IFN/GFP-RV 5‘ LTR mIFN- IRES GFP 3‘ LTR B GFP-RV IFN/GFP-RV 56% 34% 55% 35% CD 8 8% 2% 8% 2% GFP C GFP-RV IFN/GFP-RV 99% 99% CD 8 GFP Becker et al., Fig.1

2 A B C D - CD3 - CD3 - CD3 - CD3 - CD3 - peptide - peptide
IFN--/- CD8+cells Fibroblasts A B 300 Tc Tc2 20 40 60 80 100 250 200 pg IFN-  / ml pg IFN-  / ml 150 100 50 stimulation: infection: CD CD CD3 GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV IFN/GFP-RV C D IFN-+/+ CD8+cells IFN-+/+ CD8+cells 1200 1000 1000 800 800 600 pg IFN-  / ml 600 pg IFN-  / ml 400 400 200 200 CD CD3 peptide peptide stimulation: infection: GFP-RV IFN/GFP-RV GFP-RV IFN/GFP-RV Becker et al., Fig.2

3 A B C IFN GFP Infection: IFN/GFP-RV
Stimulation: unstimulated anti-CD3 Dilution: /3 1/9 1/27 -RT /3 1/9 1/27 -RT end. IFN viral IFN- total IFN GAPDH B Infection: GFP-RV Stimulation: unstimulated anti-CD3 Dilution: /3 1/9 1/27 -RT /3 1/9 1/27 -RT end. IFN viral IFN total IFN GAPDH C GFP-RV IFN/GFP-RV Unstimulated anti-CD3 IFN 58% 67% GFP Becker et al., Fig.3

4 IFN- IL-4 Tc1 Tc2 Tc1 Tc2 pg IFN-  / ml pg IFN-  / ml day 8 day16
GFP- GFP GFP - GFP + 200 400 600 800 1000 1200 1400 1600 1800 2000 pg IFN-  / ml 2000 Tc Tc2 1800 1600 1400 pg IFN-  / ml 1200 1000 800 600 400 200 GFP- GFP GFP - GFP + day day16 IL-4 500 1000 1500 2000 2500 3000 3500 4000 pg IL-4 / ml day day16 GFP- GFP GFP - GFP + Tc1 n.d. n.d n.d. n.d. 4000 Tc2 3500 3000 2500 pg IL-4 / ml 2000 1500 1000 500 GFP- GFP GFP - GFP + day day16 Becker et al., Fig.4

5 A B C V8.1, 8.2 IFN- GFP GFP GFP-RV GFP-RV 80000 70000 60000
50000 pg IFN- / ml V8.1, 8.2 IFN- IFN/GFP-RV IFN/GFP-RV 40000 30000 20000 10000 GFP GFP GFP-RV IFN/GFP-RV Becker et al., Fig.5

6 Infection Stimulation pg IFN- / ml
100 200 300 400 500 600 700 800 1000 900 GFP-RV GFP + IL-12/IL-18 IFN/GFP-RV GFP - IL-12/IL-18 IFN/GFP-RV GFP + IL-12/IL-18 Becker et al., Fig.6

7 A B % specific lysis mm mean tumor diameter Days after Challenge 1 2 3
60 50 IFN/GFP-RV + HA peptide IFN/GFP-RV GFP-RV + HA peptide GFP-RV % specific lysis 40 30 20 10 1:1 5:1 10:1 E:T ratio B 1 2 3 4 5 6 7 8 9 10 15 20 25 30 mm mean tumor diameter Days after Challenge    Becker et al., Fig.7

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