1. T+3 WL 1 4 5 7 9 3 2 6 10
kDa
76.0
66.2
43.0
36.0
31.0
21.5
17.5
3.0
5.1
5.5
5.9
6.6
7.0
4.5
10.0
8.5 pI 8 1 5
Supplementary Fig. 1.　Confirmation of the position of PR-1.2 protein by the 2-DE gel blot analysis of wheat seminal roots grown under T+3 WL conditions for 7 d. (Upper panel) 2-DE image (CBB staining) of proteins from waterlogged wheat seedlings (from Haque et al., 2011). Arrows indicate spots increased or decreased by waterlogging. (Lower panel) Proteins from wheat seedlings grown under waterlogged conditions were separated by 2-DE followed by blot analysis with an anti-rice PR-1 antibody.