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Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet.

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Presentation on theme: "Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet."— Presentation transcript:

1 Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet Digital PCR  Masaru Watanabe, Tomoya Kawaguchi, Shun-ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, Hideo Saka, Sadanori Takeo, Hirofumi Adachi, Tsutomu Tagawa, Osamu Kawashima, Motohiro Yamashita, Kazuhiko Kataoka, Yukito Ichinose, Yukiyasu Takeuchi, Katsuya Watanabe, Akihide Matsumura, Yasuhiro Koh  EBioMedicine  Volume 21, Pages (July 2017) DOI: /j.ebiom Copyright © 2017 The Authors Terms and Conditions

2 Fig. 1 Multiplex assay for analysis of EGFR mutations. Two-dimensional histogram of the 6-hexaplex assay is shown. Plasmids containing each target sequence were encapsulated in droplets and subjected to the ddPCR assay. FAM, 6-carboxyfluorescein; TET, tetrachlorofluorescein. EBioMedicine  , 86-93DOI: ( /j.ebiom ) Copyright © 2017 The Authors Terms and Conditions

3 Fig. 2 Serial dilutions of positive controls were analyzed for each EGFR mutation under study. Testing 0.04 and 4ng of genomic DNA from H1975 cells harboring EGFR mutations L858R and T790M (A) or PC-9/ZD cells harboring EGFR exon 19 deletion and substitution T790M (B); n=5. EBioMedicine  , 86-93DOI: ( /j.ebiom ) Copyright © 2017 The Authors Terms and Conditions

4 Fig. 3 Comparison of results obtained by multiplex and duplex analyses. Compilation of the analysis of FFPE samples from patients with primary tumors mutated for EGFR L858R, Exon19 deletion (Ex19del) with/without T790M mutation. EBioMedicine  , 86-93DOI: ( /j.ebiom ) Copyright © 2017 The Authors Terms and Conditions

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