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The structural basis of the cross-bridge cycle
DECORATED ACTIN was the first em reconstruction Moore, Huxley, and DeRosier 1970 J. Mol Biol.
Reconstruction by filtered least squares
Projection every 1° (181 projections) Number of observational equations is 161 x 181=29141 Order of normal matrix Problem should be well determined
Case 2. Projection every 3°, not enough data to invert the normal matrix. How much data is there? To determine the rank of a positive (semi) definite matrix we determine its eigenvalue spectrum
Decorated actin at 13Å resolving power calculated by filtered least squares
Electron cryo-microscopy (of decorated actin) shows how strong binding of myosin to actin releases nucleotide Holmes, Angert, Kull, Jahn, Schroeder (2003) Nature 425,
Four sub-domain refinement
The cross-bridge cycle As revealed by crystallography
Geeves, M.A. & Holmes, K.C. (2005) The molecular mechanism of muscle contraction Adv Protein Chem 71,
Lymn & Taylor, 1971
The myosin motor domain must move the lever arm from pre- to post- power stroke. It must bind to actin strongly or weakly Both functions are controlled by ATP binding and hydrolysis
Crystallography on the motor domain (particularly Dictyostelium Myosin 2) shows two conformations of myosin: lever UP and lever DOWN
Diskussionsthemen Nennen Sie die Hauptthesen, über die Sie sprechen werden.
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Fischer et al (2005) PNAS
Weak and Strong Binding
Coureux, P. D.Wells, A. L.Menetrey, J.Yengo, C. M.Morris, C. A.Sweeney, H. L.Houdusse, A. (2003) A structural state of the myosin V motor without bound nucleotide Nature
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