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Nanocomposite Containing Bioactive Peptides Promote Endothelialisation by Circulating Progenitor Cells: An In vitro Evaluation  N. Alobaid, H.J. Salacinski,

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Presentation on theme: "Nanocomposite Containing Bioactive Peptides Promote Endothelialisation by Circulating Progenitor Cells: An In vitro Evaluation  N. Alobaid, H.J. Salacinski,"— Presentation transcript:

1 Nanocomposite Containing Bioactive Peptides Promote Endothelialisation by Circulating Progenitor Cells: An In vitro Evaluation  N. Alobaid, H.J. Salacinski, K.M. Sales, B. Ramesh, R.Y. Kannan, G. Hamilton, A.M. Seifalian  European Journal of Vascular and Endovascular Surgery  Volume 32, Issue 1, Pages (July 2006) DOI: /j.ejvs Copyright © 2006 Elsevier Ltd Terms and Conditions

2 Fig. 1 A small proportion (1–2%) of PBMC expressed CD34 determined by fluorescence-activated cell sorter (FACS) analysis. European Journal of Vascular and Endovascular Surgery  , 76-83DOI: ( /j.ejvs ) Copyright © 2006 Elsevier Ltd Terms and Conditions

3 Fig. 2 Phase-contrast micrograph of endothelial progenitor cells (EPC) colony. European Journal of Vascular and Endovascular Surgery  , 76-83DOI: ( /j.ejvs ) Copyright © 2006 Elsevier Ltd Terms and Conditions

4 Fig. 3 Number of endothelial progenitor cells (EPC) colonies on polymers and uncoated dishes as control. Data are mean±SD. European Journal of Vascular and Endovascular Surgery  , 76-83DOI: ( /j.ejvs ) Copyright © 2006 Elsevier Ltd Terms and Conditions

5 Fig. 4 The endothelial phenotype was confirmed by immunostaining with antibodies specific for endothelial markers, (a) eNOS; (b) cell membrane and on cells border to border (arrow) CD31; (c) vWF contained within cytoplasmic granule (arrow); (d) representative control sections where nuclear staining alone is visible. All cells were counterstained with DAPI showing the nucleus as blue. European Journal of Vascular and Endovascular Surgery  , 76-83DOI: ( /j.ejvs ) Copyright © 2006 Elsevier Ltd Terms and Conditions

6 Fig. 5 AlamarBlueTM (AB) viability assay test on cells exposed to three group on day 7. Absorbance was measured in arbitrary units at 570nm wavelength and background at 630nm subtracted. Data are mean±SD. European Journal of Vascular and Endovascular Surgery  , 76-83DOI: ( /j.ejvs ) Copyright © 2006 Elsevier Ltd Terms and Conditions

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