1. Introduction to Biomedical Sensors BME 301 Biomedical Sensors Lecture Note 1 BME 301 Biomedical Sensors - Ali Işın 2014

2. What is a Biomedical Sensor? Any instrumentation system can be described as having three fundamental components: a sensor, a signal processor, and a display and/or storage device. BME 301 Biomedical Sensors - Ali Işın 2014

3. Although all these components of the instrumentation system are important, the sensor serves a special function in that it interfaces the instrument with the system being measured. In the case of biomedical instrumentation, a biomedical sensor is the interface between the electronic instrument and the biological system. BME 301 Biomedical Sensors - Ali Işın 2014

4. Important Concerns There are some general concerns that are important for any sensor in an instrumentation system interface function especially for biomedical sensors: 1. The sensor can affect the system, for that sensors must be designed to minimize their interaction with the biological host. It is important that the presence of the sensor does not affect the variable being measured in the vicinity of the sensor via interaction between the sensor and the biologic system. This may change the quantity being sensed in the vicinity. BME 301 Biomedical Sensors - Ali Işın 2014

5. 2. The biological system can affect the performance of the sensor. The foreign body reaction might cause the host’s system to attempt to break down the materials of the sensor in order to remove it. This may, in fact, degrade the sensor package so that it can no longer perform in an adequate manner. So the material of package must be proper. BME 301 Biomedical Sensors - Ali Işın 2014

6. 3. Sensors that are implanted in the body are not accessible for calibration. Thus, they must have extremely stable characteristics so that frequent calibrations are not necessary. BME 301 Biomedical Sensors - Ali Işın 2014

7. Classification of Biomedical Sensors Biomedical sensors can be classified according to how they are used with respect to the biological system: 1. Noninvasive biomedical sensors do not even contact the biological system being measured. Sensors of radiant heat or sound energy coming from an organism are examples of noncontacting sensors. Noninvasive sensors can also be placed on the body surface like Skin surface thermometers, biopotential electrodes, and strain gauges placed on the skin. BME 301 Biomedical Sensors - Ali Işın 2014

8. 2. Indwelling sensors (minimally invasive sensors) :are those that can be placed into a natural body cavity that communicates with the outside. Examples: oral–rectal thermometers, intrauterine pressure transducers, and stomach pH sensors. BME 301 Biomedical Sensors - Ali Işın 2014

9. 3. Invasive sensors: are those that need to be surgically placed and that require some tissue damage associated with their installation. BME 301 Biomedical Sensors - Ali Işın 2014

10. We can also classify sensors in terms of the quantities that they measure: 1. Physical sensors: are used in measuring physical quantities such as displacement, pressure, and flow. BME 301 Biomedical Sensors - Ali Işın 2014

11. 2. Chemical sensors: are used to determine the concentration of chemical substances within the host. A sub-group of the chemical sensors that are concerned with sensing the presence and the concentration of biochemical materials in the host. 3. Bio-analytical sensors or biosensors: used to measure some internal quantities like enzymes. BME 301 Biomedical Sensors - Ali Işın 2014

12. 1. Bio-analytical Sensors: A special class of sensors of biological molecules has evolved in recent years. These bioanalytical sensors take advantage of one of the following biochemical reactions: (1) enzyme–substrate. (2) antigen–antibody. (3) ligand–receptor. BME 301 Biomedical Sensors - Ali Işın 2014

13. The advantage of using these reactions in a sensor is that they are highly specific to a particular biological molecule, and sensors with high sensitivity and selectivity can be developed based upon these reactions. BME 301 Biomedical Sensors - Ali Işın 2014

14. The basic structure of a bio-analytical sensor; BME 301 Biomedical Sensors - Ali Işın 2014

15. There are two principal regions of the sensor. The first contains one component of the biological sensing reaction such as the enzyme or the antibody, and the second region involves a means of detecting weather the biological reaction has taken place. This second portion of a bioanalytical sensor is made up of either a physical or chemical sensor that serves as the detector of the biological reaction. This detector can consist of an electrical sensor such as used in electrochemical sensors, a thermal sensor, a sensor of changes in capacitance, a sensor of changes in mass, or a sensor of optical properties. BME 301 Biomedical Sensors - Ali Işın 2014

16. Example bio-analytical sensor: One example of a bioanalytical sensor is a glucose sensor. The first portion of the sensor contains the enzyme glucose oxidase. This enzyme promotes the oxidation of glucose to glucuronic acid and hydrogen peroxide while consuming oxygen in the process. Thus, by placing a hydrogen peroxide or an oxygen sensor along with the glucose oxidase in the bioanalytical sensor, one can determine the amount of glucose oxidized by measuring the amount of hydrogen peroxide produced or oxygen consumed. BME 301 Biomedical Sensors - Ali Işın 2014

17. Stability is important for bioanalytical sensors, especially those that are used for long-term measurements. The stability issues are also related to preservation of the biological molecules used in the first portion of the sensor. These molecules can often be degraded or destroyed by heat or exposure to light.. Thus, an important issue in dealing with bioanalytical sensors is the preservation of the biochemical components of the sensor. BME 301 Biomedical Sensors - Ali Işın 2014

18. 2.Chemical Sensors: There are many biomedical situations where it is necessary to know the concentration or chemical activity of a particular substance in a biological sample. Chemical sensors provide the interface between an instrument and the specimen to allow one to determine these quantities. These sensors can be used on a biological specimen taken from the host and tested in a laboratory, noninvasive or invasive sensors. BME 301 Biomedical Sensors - Ali Işın 2014

19. The below table indicates the most famous biomedical sensors, the most common three types of the biomedical sensors are electrochemical, optical and thermal biomedical sensors:- BME 301 Biomedical Sensors - Ali Işın 2014 ElectrochemicalOpticalThermal a.Amperometrica.Colorimetrica.Calorimetric b. Potentiometricb. Emmision and absorption spectroscopy b. Thermo conductivity c. Coulometricc. Fluorescence

20. A Clark amperometric electrode for sensing oxygen; BME 301 Biomedical Sensors - Ali Işın 2014

21. It consists of an electrochemical cell separated from the specimen being measured by an oxygen-permeable membrane. The cell is biased at a fixed potential of 600 mV, and under these conditions the following reaction occurs at the noble metal cathode: O 2 + 2H 2 O → 4e - + 4OH - BME 301 Biomedical Sensors - Ali Işın 2014

22. This reaction involves the reduction of molecular oxygen that diffuses into the cell through the oxygen permeable Membrane. Since the other components of the reaction are in abundance, the rate of the reaction is limited by the amount of oxygen available. Thus, the rate of electrons used at the cathode is directly related to the available oxygen. In other words, the cathode current is proportional to the partial pressure of oxygen in the specimen being measured. BME 301 Biomedical Sensors - Ali Işın 2014

23. The electrochemical cell is completed by the silver anode. The reaction at the anode involves forming the low-solubility salt, silverchloride, from the anode material itself and the chloride ion contained in the electrolytic solution. The cell is designed with these materials in abundance so that their activity does not affect the sensor performance. This type of sensor is an example of an amperometric electrochemical sensor that is the current in the cell is proportional to the concentration of the analyte; in this case, oxygen. BME 301 Biomedical Sensors - Ali Işın 2014

24. 3. Physical Sensors Physical variables measured include temperature, strain, force, pressure, displacement, position, velocity, acceleration, optical radiation, sound, flow rate, viscosity, and electromagnetic fields. BME 301 Biomedical Sensors - Ali Işın 2014

25. Temperature Sensors; Temperature is an important parameter in many control systems, most familiarly in environmental control systems. Several distinctly different transduction mechanisms have been employed to measure temperature. The mercury thermometer is a temperature sensor which produces a non electronic output signal. The most commonly used electrical signal generating temperature sensors are thermocouples, thermestors, and resistance thermometers. BME 301 Biomedical Sensors - Ali Işın 2014

26. Thermocouples; Thermocouples employ the Seebeck effect, which occurs at the junction of two dissimilar conductors. A voltage difference is generated between the hot and cold ends of the two conductors due to the differences in the energy distribution of electrons at the two different temperatures. The voltage magnitude generated depends on the properties of the conductor, e.g., conductivity and work function, such that a difference voltage will be measured between the cold ends of two different conductors. The voltage changes fairly linearly with temperature over a given range, depending on the choice of conductors. BME 301 Biomedical Sensors - Ali Işın 2014

27. To minimize measurement error, the cool end of the couple must be kept at a constant temperature and the voltmeter must have high input impedance. A commonly used thermocouple is made of copper and constantan wires. BME 301 Biomedical Sensors - Ali Işın 2014

28. A thermocouple is an ‘‘auto-generator,’’ i.e., it produces a usable output signal, in this case electronic, directly in response to the measurand without the need for auxiliary power. BME 301 Biomedical Sensors - Ali Işın 2014

29. Resistance thermometer; The resistance thermometer relies on the increase in resistance of a metal wire with increasing temperature. As the electrons in the metal gain thermal energy, they move about more rapidly and undergo more frequent collisions with each other and the atomic nuclei. These scattering events reduce the mobility of the electrons, and since resistance is inversely proportional to mobility, the resistance increases. Resistance thermometers typically consist of a coil of thin metal wire. Platinum wire gives the largest linear range of operation. The resistance thermometer is a ‘‘modulator’’ or passive transducer. In order to determine the resistance change, a constant current is supplied and the corresponding voltage is measured (or vice versa). A measure of the sensitivity of a resistance thermometer is its temperature coefficient of resistance (TCR). BME 301 Biomedical Sensors - Ali Işın 2014

30. Thermistors; Thermistors are resistive elements made of semiconductor materials and have a negative temperature coefficient of resistance. The mechanism governing the resistance change of a thermistor is the increase in the number of conducting electrons with increasing temperature, due to thermal generation, i.e., the electrons that are the least tightly bound to the nucleus (valence electrons) gain sufficient thermal energy to break away (enter the conduction band) and become influenced by external fields. Thermistors are measured in the same manner as resistance thermometers, but thermistors have up to 100 times higher TCR values. BME 301 Biomedical Sensors - Ali Işın 2014

31. Displacement and Force Sensors; Many types of forces can be sensed by the displacements they create. For example, the force due to acceleration of a mass at the end of a spring will cause the spring to stretch and the mass to move. Its displacement from the zero acceleration position is governed by the force generated by the acceleration (F =m · a) and by the restoring force of the spring. BME 301 Biomedical Sensors - Ali Işın 2014

32. Another example is the displacement of the center of a deformable membrane due to a difference in pressure across it. Both of these examples require multiple transduction mechanisms to produce an electronic output: a primary mechanism which converts force to displacement (mechanical to mechanical) and then an intermediate mechanism to convert displacement to an electrical signal (mechanical to electrical). BME 301 Biomedical Sensors - Ali Işın 2014

33. Applications Of Biomedical Sensors: Biomedical research: One of the application fields of the biomedical sensors is using them in continuous biomedical research. So, these sensors can be used to improve the quality of the biomedical product). BME 301 Biomedical Sensors - Ali Işın 2014

34. Patient care applications: Sensors are used as a part of instruments that carry out patient monitoring by making measurements such as blood pressure, oxygen saturation, body temperature and ECG. BME 301 Biomedical Sensors - Ali Işın 2014

35. Specimen analysis: This can include analyses that can be carried out by the patients themselves in their homes such as it is done with home blood glucose analyzers. Sensors also are a part of large, multi- component, automatic blood analyzers used in the central clinical laboratories of medical centers. BME 301 Biomedical Sensors - Ali Işın 2014